摘要
以宁杞1号苗木为材料,采用OTC气室控制CO_2浓度,设置自然环境CO_2浓度(350±20μmol·mol^(-1),CK)和倍增CO_2浓度(700±20μmol·mol-1,TR)2个处理,测定宁夏枸杞果实中蔗糖代谢相关酶活性的变化,研究大气CO_2浓度倍增对宁夏枸杞果实糖代谢的影响。结果表明:在高浓度CO_2下,夏果中(CO_2处理90d),合成酶类和分解酶类酶活性均为处理组>对照组,其中转化酶(AI、NI)活性差异极显著;秋果中(CO_2处理120d)合成酶类与分解酶类较夏果活性均有降低,分解酶类活性为处理组>对照组,合成酶类活性为处理组<对照组,且其中SS合成方向差异极显著。据此,AI、NI及SS(合成方向)可能是高浓度CO_2下蔗糖代谢响应的关键酶,且长期高浓度CO_2处理会降低宁夏枸杞果实中蔗糖代谢相关酶的活性。
Taking Lycium barbarum seedlings of cultivar ＂Ningqi No.1＂as research materials,effects of high atmospheric CO_2 concentration on the activities of the enzymes related to sucrose metabolism were investigated by measuring the activities of sucrose metabolism-related enzymes in L.barbarum fruit.Two CO_2 concentrations were set up：natural environment with a CO_2 concentration of（350±20）μmol·mol-1（control）and doubled CO_2concentration（700±20）μmol·mol^（-1） generated by open top chamber.The results showed that under high CO_2 concentration,the activities of the enzymes on sucrose synthesis and decomposition in summer fruit 90 dafter treatment were higher than those in the control,in which differences in invertase（AI,NI）activity was significant.While in autumn fruit 120 dafter treatment,the activities of the enzymes on sucrose synthesis and decomposition were lower than those in summer fruit,the activities of the enzymes on sucrose decomposition were higher than those in the control,the activities of the enzymes on sucrose synthesis were lower than those in the control,and the differences in SS（synthetic direction）were significant.It was concluded that the acid invertase（AI）,the neutral invertase（NI）and sucrose synthase（SS synthetic direction）activities might be the key factors of sucrose metabolism-related under doubled atmospheric CO_2 concentrations.Moreover,the activities of sucrose metabolism-related enzymes would decrease under long-term doubled atmospheric CO_2 concentration.
出处
《西北林学院学报》
CSCD
北大核心
2016年第4期44-47,105,共5页
Journal of Northwest Forestry University
基金
宁夏大学研究生科技创新项目(GIP2015023)
国家自然科学基金项目(30860227
31160172)
关键词
宁夏枸杞
果实
CO2浓度倍增处理
蔗糖代谢相关酶
Lycium barbarum
fruit
doubled atmospheric CO2concentrations
sucrose metabolism-related enzymes
