摘要
目的比较大鼠骨髓和脂肪来源的间充质干细胞在2型糖尿病中的疗效。方法 3 0只成模2型糖尿病大鼠随机分为3组:糖尿病组(T2DM,n=10)、骨髓间充质干细胞治疗组(BMSC,n=10)及脂肪间充质干细胞治疗组(ADSC,n=10),同期正常大鼠(n=10)作为对照组。干细胞分别从正常大鼠骨髓及腹股沟脂肪组织分离培养获得。制模后和细胞注射后每日检测各组血糖。细胞注射后第7天行空腹糖耐量和胰岛素敏感性实验。胰腺组织行胰岛素/胰高血糖素免疫荧光染色。结果与T2DM组比较,干细胞注射7d后2型糖尿病大鼠的随机血糖持续缓慢下降,糖耐量及胰岛素敏感性均得以改善,胰岛素抵抗指数(HOMA-IR)降低,胰岛内β细胞数量增加(P<0.05),但是两种类型的间充质干细胞的疗效并无显著差异。结论骨髓和脂肪来源的间充质干细胞在2型糖尿病大鼠中的疗效相当,脂肪间充质干细胞可作为2型糖尿病治疗的理想细胞。
Objective To compare the effects on type 2 diabetes of mesenchymal stem cells（MSCs） derived from bone marrow and adipose tissue.Methods Thirty type 2 diabetic rat models were established by an eight weeks high-fat diet（HFD） with a low dose streptozotocin（STZ,25mg/kg）,and randomly assigned into three groups（10 each）：diabetes group（T2DM）,bone marrow MSCs transplantation group（BMSC） and adipose tissue MSCs transplantation group（ADSC）.Ten normal rats were set as control.MSCs were isolated from bone marrow or inguinal adipose tissue of normal rats.One week after STZ injection,3×106 MSCs suspended in 1ml PBS were infused into rats via tail vein.The blood glucose was measured every day after MSCs transplantation,the intraperitoneal glucose tolerance test（IPGTT） and intraperitoneal insulin tolerance test（IPITT） were performed the 7th day after transplantation to evaluate the effects of MSCs on diabetic rats.Pancreatic tissues were collected for insulin/glucagon immunofluorescence staining.Results After MSCs transplantation,the blood glucose decreased gradually and continuously in type 2 diabetic rats,with glucose tolerance and insulin sensitivity improved greatly.The improved insulin sensitivity was further confirmed by a decreased HOMA-IR（homeostasis model of assessment for insulin resistance） index and increased pancreas islet β-cells（P〈0.05）.However,no significant differences were observed between BMSC and ADSC group.ConclusionBoth BMSC and ADSC have the same effect on type 2 diabetic rats,so the ADSC will be the ideal stem cells for treatment of type 2 diabetes.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2016年第7期534-538,共5页
Medical Journal of Chinese People's Liberation Army
基金
国家高技术研究发展计划(8 6 3计划)项目(2013AA020105)~~
关键词
间质干细胞
糖尿病
胰岛素抗药性
胰岛素分泌细胞
mesenchymal stem cells
diabetes mellitus
insulin resistance
insulin-secreting cells
