摘要
利用双水相萃取技术提取分离油楠种子中的总皂苷,以乙醇/硫酸铵为双水相萃取体系,研究乙醇质量分数、硫酸铵质量分数、p H值、温度等因素对萃取效果的影响。结果表明:在乙醇质量分数为30%,硫酸铵质量分数为20%,p H6.0,温度为30℃的萃取条件下,油楠总皂苷在两相的分配系数为12.60,回收率为94.8%。该方法可用于油楠种子中总皂苷的提取分离。
The ethyl alcohol/ammonium sulfate aqueous two-phase system was used to extract the total saponins from Sindora glabra seeds. Effectes of the mass fraction of ethyl alcohol,mass fraction of ammonium sulfate,p H,temperature on the extraction of saponins were studied. The results indicate that the better condictions were obtained as the mass fraction of ethyl alcohol was 30%,mass fraction of ammonium sulfate was 20%,p H was 6,temperature was 30 ℃,partition coefficient of the total saponins in the two-phase system was 12. 60,the recovery rate was 94. 8%. The method is suitable for extraction and separation of saponins from Sindora glabra seeds.
出处
《热带生物学报》
2016年第2期270-273,共4页
Journal of Tropical Biology
基金
海南省高等学校科研基金项目(Hjkj2013-04)
海南省中西部高校提升综合实力项目(201328)
关键词
油楠种子
总皂苷
双水相萃取
双水相体系
Sindora glabra seeds
total saponins
ATPE（aqueous two-phase extraction）
aqueous two-phase system
