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响应面分析法优化新疆两色金鸡菊中总黄酮提取条件 被引量:3

Optimization of Extraction for Flavonoids from Coreopsis Tinctoria Nutt.by Response Surface Methodology
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摘要 目的采用响应面分析法(RSM)优化超声波提取新疆两色金鸡菊中总黄酮的提取条件。方法采用BoxBehnken中心组合设计及RSM分析影响新疆两色金鸡菊中总黄酮的超声波提取条件。结果经RSM数据处理,获得最佳提取条件为:超声提取30 min,液料比21∶1,乙醇浓度60%。在该条件下,新疆两色金鸡菊中总黄酮平均提取率(4.65±0.036)%(n=3)。结论在最佳提取条件下,可从新疆两色金鸡菊中获得质量稳定、含量较高的总黄酮提取物。 Objective Response surface methodology ( RSM ) was applied to optimize the ultrasonic extraction conditions for flavonoids from Coreopsis tinctoria Nutt. Methods The influence factors of ultrasonic extraction were evaluated using the Box-Behnken central component experiments and analyzed by RSM. Results The optimum extraction conditions were confirmed as follows:extraction time 30. 0 min, ratio of liquid to solid 21∶1, concentration of ethanol 60%. The yield of flavonoids under this condition was (4.65±0.036)% (n=3). Conclusion The flavonoids could be extracted with stability and higher yield from Coreopsis tinctoria Nutt under optimized conditions.
出处 《医药导报》 CAS 2016年第7期765-768,共4页 Herald of Medicine
基金 新疆生产建设兵团重点领域科技攻关项目(2014BA031) 石河子大学高层次人才科研启动项目(RCZX201328)
关键词 两色金鸡菊 总黄酮 响应面分析法 Coreopsis tinctoria Nutt. Flavonoids Response surface method
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