摘要
研究竹叶黄酮对异氟醚吸入诱发老年大鼠神经细胞凋亡及认知功能障碍的影响.分离培养原代海马神经细胞,暴露于体积分数为2%异氟醚6 h后,分别给予50、80及100 mg/L竹叶黄酮处理,MTT法检测细胞增殖能力,流式细胞仪检测细胞凋亡情况.大鼠经吸入1.4%体积的异氟醚2 h后,分别于1-5 d连续腹腔注射25、50、100 mg/kg竹叶黄酮,于第6天进行水迷宫实验检测大鼠空间学习记忆能力,ELISA法检测海马组织匀浆中的Aβ1-42、TNF-α、INF-γ含量.结果发现,竹叶黄酮可显著促进海马神经细胞的增殖,并且抑制异氟醚所致的海马神经细胞凋亡.与异氟醚单独处理组相比较,各浓度竹叶黄酮可显著降低大鼠逃避潜伏期并增加大鼠过台次数,ELISA结果显示竹叶黄酮可显著抑制海马组织中Aβ1-42、TNF-α及INF-γ的表达.表明竹叶黄酮可增强大鼠抗炎能力,抑制海马组织中Aβ1-42的产生,从而抑制异氟醚所致的老年大鼠神经损伤及认知功能障碍.
To examine the effect of bamboo leaf flavonoids on isoflurane-induced neuronal apoptosis and cognitive impairment in aged rats.The primary hippocampal neurons were isolated and cultured.After hippocampal neurons were treated with 2% isoflurane for 6 h,and treated with 50,80 and 100 mg/L of bamboo leaf flavonoids,the cell proliferation was measured by MTT method and cell apoptosis was measured by flow cytometry.The rats were inhaled with 1.4% isoflurane for 2 h,and injected intraperitoneally with 25,50 and100 mg/kg of bamboo leaf flavonoids for 5 consecutive days.The cognitive functions were examined by Morris water maze.The hippocampus were isolated for the determinations of Aβ1-42,TNF-α and INF-γ by ELISA.Results showed that bamboo leaf flavonoids significantly increased the proliferation of hippocampal neurons and suppressed hippocampal neurons apoptosis compared with isoflurane treatment only.The result of Morris water maze showed that bamboo leaf flavonoids significantly decreased average escape latent period and increased the frequency of crossing platform.The results of ELISA showed that bamboo leaf flavonoids suppressed the expression of Aβ1-42,TNF-α and INF-γ in hippocampus.Our findings suggested that bamboo leaf flavonoids enhanced immunity of aged rats and suppressed the expression of Aβ1-42,leading to the inhibition of isoflurane-induced neuronal apoptosis and cognitive impairment.
出处
《生物化学与生物物理进展》
SCIE
CAS
CSCD
北大核心
2016年第7期699-705,共7页
Progress In Biochemistry and Biophysics
关键词
竹叶黄酮
异氟醚
细胞凋亡
认知功能
bamboo leaf flavonoids
isoflurane
cell apoptosis
cognitive impairment
