摘要
目的研究溶血磷脂酸(LPA)对体外培养恒河猴视网膜色素上皮细胞增殖活力、超微结构及吞噬功能的影响。方法对照组细胞用无血清EMEM/F12培养液培养,实验组加入10 mmol·L^(-1)溶血磷脂酸。用MTT法检测溶血磷脂酸体外培养的第3代恒河猴视网膜色素上皮细胞增殖活力;流式细胞术检测细胞周期并固定进行透射电镜检查。将细胞培养至第3代无色素后,加入外源色素并用酶联免疫检测仪定量分析吞噬能力。结果实验组吸光度明显高于对照组(P<0.05)。实验组G1期细胞数低于对照组(P<0.01),S期高于对照组(P<0.01)。实验组恒河猴视网膜色素上皮细胞吞噬功能与对照组相比,差异无统计学意义(P>0.05)。对照组与实验组细胞超微结构无明显异常改变。结论溶血磷脂酸同时作用于恒河猴视网膜色素上皮细胞周期的G1→S转折点和G2→M转折点,促进恒河猴视网膜色素上皮细胞的增殖,但是对细胞的超微结构和吞噬功能没有明显影响。
Objective To investigate the effect of lysophosphatidic acid（ LPA） on the cell ultrastructure,proliferation and phagocytosis of rhesus retinal pigment epithelialium cells（ rRPE） in vitro. Methods Adult rRPE cells were harvested by enzyme dispase. The primary rRPE cells was cultured and passaged in vitro and observed by transmission electron microscopy. The proliferation of rRPE cultured with LPA was observed by MTT. The cell cycle of rRPE was observed by flow cytometry and detected by transmission electron microscope. And the exogenous pigment in the third cultured rRPE was detected by enzyme linked immunoassay.Results The absorbance of test group was higher（ P〈0. 05） than that of control group from the second day. The cell number in G1 phase of test group was less than that of control group（ P〈0. 01）,but the cell number in S phase was higher than that of control group（ P〈0. 01）. The phagocytosis of rRPE in two groups had no statistical difference（ P〈0. 05）. The ultrastructure changes of cell injury were not observed by transmission electron microscopy in two groups. Conclusion LPA promoted the cell proliferation of rRPE obviously. LPA promoted DNA synthesize in S phase and cell devision in G2 / M phase. But LPA had no significant effect on the ultrastructure and phagocytic function of the cells.
出处
《中国临床药理学杂志》
CAS
CSCD
北大核心
2016年第13期1220-1222,1239,共4页
The Chinese Journal of Clinical Pharmacology
基金
福建省自然科学基金资助项目(2014J01307)
关键词
恒河猴
视网膜色素上皮细胞
溶血磷脂酸
细胞增殖
细胞周期
rhesus
retinal pigment epithelialium cell
lysophosphatidic acid
cell proliferation
cell cycle
