摘要
目的:探讨袖状胃切除术对2型糖尿病大鼠骨骼肌葡萄糖转运蛋白4(Glucose transporters 4,GLUT4)表达的影响。方法:将30只2型糖尿病建模成功的大鼠分为空白对照组、假手术组和手术组,检测术前及术后第1、4、8、12周各组大鼠体质量、空腹血糖,术后第12周测定糖耐量、血清胰岛素的水平。取大鼠骨骼肌组织,采用荧光实时定量PCR技术检测骨骼肌组织中GLUT4 mRNA的表达。结果:与假手术组和空白对照组比较,手术组大鼠术后第1周体质量开始下降(p<0.05)、空腹血糖逐渐降低(p<0.05)。术后12周口服糖耐量实验明显改善、胰岛素水平升高、胰岛素抵抗指数降低、骨骼肌组织中GLUT4 mRNA的表达显著升高(p<0.01)。结论:袖状胃切除术能改善2型糖尿病大鼠糖代谢可能与提高骨骼肌组织中GLUT4 mRNA的表达、缓解胰岛素抵抗有关。
Objective： To investigate the effect of sleeve gastrectomy on glucose transporters 4 （GLUT4） expression in type 2 diabe- tid rat skeletal muscle. Methods： Thirty type 2 diabetid rats were randomly divided into three groups： control group, sham surgery group, and sleeve gastreetomy surgery group. The body of the quality and fasting plasma glucose were measured before surgery and 1, 4, 8, 12 weeks after surgery in all groups. The body of the quality, fasting plasma glucose, glucose tolerance index and serum insulin level were detected on the twelfth week after the surgery. Skeletal muscle were harvested 12 weeks postoperatively and Real - time fluorescent quantitative PCR was used to detect GLUT4 mRNA expression in skeletal muscle. Results： The body weight of rats and fasting glucose in surgery group were significantly lower than that in control group and sham surgery group 1 week after surgery （p 〈 0.05）. 12 weeks after surgery, oral glucose tolerance test significantly was improved, insulin levels was higher, insulin resistance index was lower, the expression of GLUT4 mRNA in the skeletal muscle was significantly higher in surgery group than that in control group and sham surgery group（p 〈 0.01 ）. Conclusion： Sleeve gastrectomy could improve glycometabolism in type 2 diabetid rats, which may be associated with the increase of the expression of GLUT4 mRNA in the skeletal muscle and relief of insulin resistance.
出处
《内蒙古农业大学学报（自然科学版）》
CAS
2016年第2期1-5,共5页
Journal of Inner Mongolia Agricultural University(Natural Science Edition)
基金
内蒙古自治区高等学校科学研究项目(NJZY099)
关键词
2型糖尿病
袖状胃切除术
胰岛素抵抗
骨骼肌
GLUT4
Type 2 diabetes mellitus
sleeve gastrectomy
insulin resistance
skeletal muscle
glucose transporters 4
