摘要
目的制定＂三伏贴＂凝胶膏剂的质量标准。方法采用薄层色谱法（TLC）对方中的麻黄、延胡索进行定性鉴别,运用高效液相色谱（HPLC）法对制剂中的芥子碱硫氰酸盐进行含量测定。结果 TLC斑点清晰,分离度良好,阴性对照无干扰;芥子碱硫氰酸盐在0.0109～0.0872mg/m L与峰面积分值呈良好的线性关系（r=1.0000）,平均加样回收率为98.33%,RSD=2.01%.结论所建立的定性定量方法分离度好,简便,专属性好,可用于三伏贴凝胶膏剂的质量控制。
Objective To establish the quality standard of dog-days paste cataplasm. Methods Qualitative identification of Ephedra, Corydalis Rhizoma in the formula was carried out by thin-layer chromatography （TLC）. High performance liquid phase chromatography （HPLC） was used for the content of sinapine thiocyanate in the preparation. Results TLC spots were clear and wellseparated without negative interference, the line range of sinapine thiocyanate was 0.0109-0.0872mg/mL, respectively, with good linear relationship with the peak area integral value（r=1.0000）, the average recovery rate was 98.33%, RSD was 2.01%. Conclusion The qualitative and quantitative method with good resolution and was simple, convinient, and reliable with good precision, It can be used for the quality control of dog-days paste cataplasm.
出处
《云南中医学院学报》
2016年第3期22-25,共4页
Journal of Yunnan University of Traditional Chinese Medicine
基金
2014年银川市科技发展计划(2014057)
