摘要
目的探讨杜仲总黄酮对铅中毒小鼠免疫器官抗氧化能力的影响。方法将50只雄性小鼠分为正常对照组、铅中毒组、阳性对照组及杜仲总黄酮治疗组(高、低剂量分别为200、50 mg/kg)。连续8 d腹腔注射70 mg/kg乙酸铅溶液,复制铅中毒小鼠模型。杜仲总黄酮治疗14 d后,摘眼球取血及脾脏和胸腺,测定血液、脾脏、胸腺中铅的含量,检测脾脏和胸腺指数及脾脏和胸腺中总超氧化物歧化酶(TSOD)及谷胱甘肽过氧化物酶(GSH-Px)活性,总抗氧化能力(T-AOC)以及丙二醛(MDA)含量。结果连续8 d腹腔注射70 mg/kg乙酸铅溶液造成铅中毒小鼠免疫能力下降,杜仲总黄酮可明显提高铅中毒小鼠的免疫能力,主要表现为连续灌胃杜仲总黄酮14 d后,铅中毒小鼠脏器指数明显提高(P<0.01)。与铅中毒组相比,200mg/kg杜仲总黄酮治疗组铅中毒小鼠血液、脾脏和胸腺中铅的含量显著降低,脾脏和胸腺中SOD、GSH-Px活性及T-AOC提高,MDA含量降低(P<0.01);50 mg/kg杜仲总黄酮治疗组铅中毒小鼠血液、脾脏和胸腺中铅的含量降低不明显,但脾脏和胸腺中SOD、GSH-Px活性及T-AOC均有不同程度地提高,MDA含量降低。结论杜仲总黄酮可有效增强铅中毒小鼠免疫器官的抗氧化能力。
Objective To explore the effects of total flavonoids of Eucommia ulmoides on antioxidant ability of immune organs in lead poisoning mice. Methods Fifty male mice were randomly divided into 5 groups,normal control group,lead group,positive control group,the total flavonoids of Eucommia ulmoides groups（ 200,50 mg / kg）. The lead poisoning model was established by peritoneally injecting mice with 70 mg / kg lead acetate every day for 8 days. After the total flavonoids of Eucommia ulmoides was gavaged for 14 days,mice were sacrificed and immediately subjected to necropsy. The concentration of lead in blood,spleen and thymus were determined. The spleen and thymus index and the content of T-SOD、GSH-Px、T-AOC and MDA of spleen and thymus in mice were assayed.Results Intraperitoneal injection of 70 mg / kg lead acetate for 8 consecutive days could cause an immunity decline in lead poisoning mice,the total flavonoids of Eucommiaulmoides could significantly improve the immunity of lead poisoning mice. After the total flavonoids of Eucommia ulmoides was gavaged for 14 days,the viscera index in mice induced by lead acetate was significantly improve（ P〈0. 01）. Compared with lead group,the total flavonoids of Eucommia ulmoides（ 200 mg / kg） could significantly reduce the lead contents in blood,spleen and thymus and improve the activities of T-SOD、GSH-Px、TAOC and reduce the content of MDA of mouse spleen and thymus（ P〈0. 01）,while there were no significant changes in concentrations of the lead contents in blood,spleen and thymus in the total flavonoids of Eucommia ulmoides（ 50 mg / kg） treatment group. But the total flavonoids of Eucommia ulmoides（ 50 mg / kg） could increase the activities of T-SOD、GSH-Px、T-AOC and reduce the content of MDA of mouse spleen and thymus in different degree. Conclusion The total flavonoids of Eucommia ulmoides can effectively improve the antioxidant ability of immune organs in mice induced by lead acetate.
出处
《卫生研究》
CAS
CSCD
北大核心
2016年第4期643-647,共5页
Journal of Hygiene Research
基金
国家自然科学基金(No.31560640)
吉首大学植物资源开发与利用产学研合作示范基地产业化培育项目(No.13JDCY05)
关键词
杜仲总黄酮
铅中毒
脏器指数
抗氧化能力
total flavonoids of Eucommia ulmoides
lead poison
viscera index
antioxidant ability
