摘要
目的通过对本实验室开展的抗核抗体(ANA)和特异性抗核抗体谱(ANAs)的分析研究,探讨二者之间的相关性。方法采用间接免疫荧光法(IIF)检测ANA,免疫印迹法检测ANAs,对1 048例ANA阳性标本进行2种方法的比对分析。结果 1 048例ANA阳性标本中,核颗粒型占44.9%;核均质型占23.3%;胞浆颗粒型占11.8%;着丝点型占8.9%;核仁型占4.3%;高尔基体型占2.3%;核周型占1.5%;中心粒型占0.9%;核点型占1.1%;胞浆纤维型占0.9%。主要核型为核颗粒型和核均质型。ANAs的阳性率为60%。所有荧光核型均以抗SS-A抗体阳性为多,其中n RNP/Sm、Sm、SS-A、SS-B、Ro-52特异性抗体的荧光核型以核颗粒型为主;抗nukleosome、his-tones抗体的核型主要是核均质型;胞浆颗粒型中的主要特异性抗体为n RNP/Sm、SS-A、Ro-52;抗CENP B对着丝点核型的诊断具有较高的特异性。结论 ANA检测的荧光核型与ANAs特异性抗体之间有一定的相关性,但仍存在不一致性。必须联合检测才能提高检出率,在AID的诊断中发挥一定的互补作用。
To evaluate the correlation between the antinuclear antibodies （ANA） and the specific antinuclear antibodies spectrum （ANAs）, total of 1048 ANA positive samples were collected and analyzed. ANA was detected by the indirect immunofluorescence assay （IIF）, while ANAs was detected by the immunoblotting respectively, and then, the comparison of two methods for 1 048 ANA positive samples was performed. In 1048 eases of ANA positive samples, speckled pattern type accounted for 44.9%, homogeneous pattern type accounted for 23.3%, cytoplasmic pattern type accounted for 11.8% , centromere pattern type accounted for 8.9% , nucleolar pattern type accounted for 4.3%, Golgi body pattern type accounted for 2.3%, perinuclear pattern type accounted for 1.5%, central grain pattern type accounted for 0.9% , nuclear dots pattern type accounted for （1.1%） and cytoplasmic fiber pattern type accounted for 0.9%. The main karyotype patterns were speckled pattern and homogeneous pattern. The positive rate of ANAs was 60%. Of all the karyotype patterns, the most common specific antibody was anti-SSA. The karyotype pattern of anti-nRNP/Sm, anti-Sm, anti-SS-A, anti-SS-B, and anti-Ro-52 specific antibodies was speckled pattern mainly. The karyotype pattern of anti-histone and anti-nucleosome antibodies was homogeneous pattern mainly. The main specific antibodies in cytoplasmic pattern were anti-nRNP/Sm, anti-SS-A and anti-Ro-52. Anti-CenpB antibody was specific to centromere pattern. There was a certain correlation between the ANA and the ANAs, but there was also inconsistency. Taken together, the combined detection can improve the detection rate and play a complementary role in the diagnosis of AID.
出处
《免疫学杂志》
CAS
CSCD
北大核心
2016年第8期733-736,共4页
Immunological Journal
关键词
抗核抗体
间接免疫荧光
抗核抗体谱
免疫印迹
Antinuclear antibody
Indirect immunofluorescence
Antinuclear antibody spectrum
Immunoblotting
