摘要
CRISPR/Cas(Clustered regularly interspaced short palindromic repeats(CRISPR)/CRISPR-associated(Cas)系统是由一种短小RNA调控的对DNA修饰的基因编辑工具,是一种较锌指核酸酶(Zinc-fingers nuclease,ZFN)和转录激活因子样效应物核酸酶(Transcription activator-like(TAL)effector nucleases,TALEN)打靶更加快速、高效、精准的新型基因组编辑工具,它易于设计,且具有特异性。本文回顾了CRISPR/Cas系统的结构与功能,概述了Cas9的设计策略、影响Cas9基因编辑效率的因素、脱靶检测分析方法及其在动物基因编辑研究中的应用。基于CRISPR/Cas9已经在多种动物上成功实现了基因编辑,它有望成为建立动物模型和研究疾病防治的一种新型可行性途径。
CRISPR/Cas（Clustered regularly interspaced short palindromic repeats（CRISPR）/CRISPR-associated（Cas））system is a DNA modified gene editing tools regulated by a kind of short RNA.This technology is a novel tool,which is more rapid,efficient and accurate than ZFN（zinc-fingers nuclease）and TALEN（transcription activator-like（TAL）effector nucleases）in genetic engineering.It is easy to be designed and has specificity.This paper reviews the structure and function of CRISPR/Cas9 system and the design strategy of Cas9 and its application in animal genetic engineering.CRISPR/Cas9 is successfully used in a variety of animal genetic editing,it is likely to become a new way to establish animal models and to develop a new feasible approach to prevent disease.
出处
《畜牧兽医学报》
CAS
CSCD
北大核心
2016年第7期1299-1305,共7页
ACTA VETERINARIA ET ZOOTECHNICA SINICA
基金
陕西省农业科技创新与攻关项目(2014K02-05-01)
2015年西北农林科技大学第二批基本科研业务费科技创新专项(Z109021566)
