摘要
目的建立HPLC法同时测定拟缺香茶菜不同入药部位中布卢门醇A、尾叶香茶菜丙素、鄂西香茶菜素含量的方法。方法色谱柱：YMC C18（4.6 mm×250 mm,5μm）反相色谱柱;流动相：乙腈-水（32∶68）等度洗脱;流速：1.0 m L/min;柱温：30℃;检测波长：230 nm。结果布卢门醇A、尾叶香茶菜丙素、鄂西香茶菜素分别在0.001 1~0.022 2 g/L（r=0.999 7）,0.001 9~0.037 1g/L（r=0.999 5）,0.013 3~0.265 g/L（r=0.999 7）范围内呈良好的线性关系;平均回收率分别为99.58%（RSD=2.29%）,99.51%（RSD=1.65%）,102.78%（RSD=1.57%）。不同产地拟缺香茶菜不同部位中3个活性成分的含量均以叶中最高。结论所建立的方法灵敏、准确、稳定,可用于拟缺香茶菜不同部位中3个活性成分的含量测定。
Objective To establish the method of simultaneous determination of blumenolA,kamebanin and henryin in different parts of Isodon excisoides by using HPLC.Methods The parameters of HPLC were listed as the following：YMC C18（4.6 ＆#215;250 mm,5 μm）reversed-phase LC column;acetonitrile-water （32∶68）isocratic elution;flow rate at 1 .0 mL/min;temperature of 30 ℃;UV detection wave-lengthof230nm.Results BlumenolA,kamebanin and henryin showed a good linearity in the range of 0.001 11 ~0.022 2 g/L （r=0.999 7）,0.001 855 ~0.037 1 g/L （r=0.999 5）,0.013 25 ~0.265 0 g/L （r=0.999 7）respectively.The average recovery of the three ingredients were 99.58% （blumenol A）,99.51% （kamebanin）and 102.78% （henryin）with RSD 2.29%,1.49%,1.57% correspond-ingly.The content of three index components in different parts of Isodon excisoides varied,with the largest contentinleaves.Conclusion Themethodestablishedissensitive,accurateandeffective,whichcan be used for determination of the content of three active components in different parts of Isodon excisoides.
出处
《北京中医药大学学报》
CAS
CSCD
北大核心
2016年第6期494-497,501,共5页
Journal of Beijing University of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(No.81173486)
河南省青年骨干教师资助项目(No.2012GGJS-095)
河南省中医药管理重点项目(No.2013ZY02063)
省属高校基本科研业务经费项目(No.2014KYYWF-QN02)
河南中医药大学科技创新人才资助项目(No.2015XCXRC04)
