摘要
目的研究巨噬细胞刺激因子(macrophage stimulating factor,MSF)及巨噬细胞抑制因子(macrophage inhibitory factor,MIF)对视网膜节细胞(retinal ganglion cell,RGC)轴突再生的影响。方法实验性研究。费希尔大鼠9只,施行视神经损伤术7 d后取出眼球并剥离视网膜,把剥离下来的视网膜平铺,放射状剪成8片,每一片视网膜粘铺于已包被好的培养板孔中,分为对照组、MSF组、MIF组,分别加入培养基及MSF/MIF等处理因素试剂;培养7 d后对视网膜培养块进行固定及免疫荧光染色,分别计数RGC再生神经轴突的数量及长度和外迁巨噬细胞的数量。结果与对照组相比,MSF及MIF可以一定程度上分别增多及减少迁移巨噬细胞的数量(P>0.05),与此同时,MIF可以减少RGC再生神经轴突的数量及长度(P<0.05),MSF虽未能增加神经突的长度,但还是有效地增加了再生神经突的数量(P<0.05)。结论 MSF可以促进RGC神经轴突的再生,与此相反MIF可以抑制神经轴突的再生。
OBJECTIVE To investigate the effects of macrophage stimulating factor（MSF） and macrophage inhibitory factor（MIF） on retinal ganglion cells（RGC） axon regeneration in retinal explant. METHODS It was an experimental study. Seven days after optic nerve（ON） transection of Fischer rat, retinas were extracted out and flat-mounted onto a filter paper. After being cut into 8 pieces, each piece of retina was glued to the substrate of culture plate and incubated in culture medium with MSF or MIF interventions respectively. Retinal explants were fixed after cultured for seven days. After fluorescent immunostaining, migrating out macrophages and regenerating axon were counted under the fluorescent microscope. Main outcome were number of migrating out macrophage, number and length of regenerating axons. RESULTS Compared with the control group, MSF and MIF could increase or decrease the number of migrating out macrophages respectively. Meanwhile, MIF could increase the number of regenerating axons and their length. However, MSF could only increase the number of regenerating axons, but had no effect on axon length. CONCLUSIONS The MSF could promote axon regeneration in retinal explant, while MIF could inhibit axon regeneration.
出处
《中国中医眼科杂志》
2016年第3期150-153,共4页
China Journal of Chinese Ophthalmology
基金
国家自然科学研究基金(81570849)
高等学校博士学科点专项科研基金(20114402120007)
广东省自然科学基金(2015A030313446)
关键词
视网膜节细胞
巨噬细胞
视神经损伤
存活
神经再生
retinal ganglion cell
macrophage
injury of optic nerve
subsist
axon regeneration
