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2型糖尿病合并巨细胞病毒感染患者中性粒细胞表面黏附分子 CD11b表达及临床意义

Expression and significance of neutrophil surface adhesion molecules CD11b in type 2 diabetes mellitus patients with cytomegalovirus infection
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摘要 目的:探讨2型糖尿病患者并发巨细胞病毒(CMV)感染后中性粒细胞表面黏附分子 CD11b的表达及诊断价值。方法收集2型糖尿病患者共139例,其中男99例、女40例,同时选取门诊体检健康人群男20例、女13例作为健康对照组。通过 CMV-pp65抗原检测将2型糖尿病患者分为 CMV 高活动性感染组、CMV 活动性感染组和非 CMV 感染阴性对照组,同时选取健康人作为正常对照组。采用流式细胞仪分别检测上述各组的中性粒细胞表面黏附分子 CD11b 表达比例。计算 CD11b 在诊断2型糖尿病并发 CMV 感染组的受试者工作曲线(ROC 曲线)。结果2型糖尿病合并 CMV 高活动性感染者体内中性粒细胞表达 CD11b的比例为(41.25±5.33)%,与 CMV 活动性感染组的(61.13±5.28)%差异有统计学意义(P =0.019),与健康对照组的(64.21±6.02)%相比差异有统计学意义(P =0.009)。CD11b 在诊断2型糖尿病并发 CMV 高活动性感染组的最佳临界值为表达率45.65%,敏感度为100.00%,特异度为60.12%,受试者工作曲线下面积为0.628;CD11b 诊断 CMV 活动性感染组的最佳临界值为表达率70.21%,敏感度为87.69%,特异度为99.22%,受试者工作曲线下面积为0.991。结论2型糖尿病并发 CMV 感染后中性粒 CD11b 表达下调,可以作为鉴别诊断 CMV 活动性感染的实验室依据。 Objective To study diagnosis significance of neutrophil surface adhesion molecules including CD11b,in type 2 diabetes mellitus patients with cytomegalovirus (CMV)infection.Methods Blood samples from 139 cases of type 2 diabetes mellitus patients were collected including 99 male and 40 female cases between Septem-ber 2011 and January 2015.Healthy male 20 cases and female 13 cases were collected as healthy control group from outpatients in the same period.Type 2 diabetes mellitus patients were divided into high active CMV infection group, CMV infection group and the negative control group,and healthy group as normal control group according to CMV -pp65 antigen detection.Neutrophil surface adhesion molecule CD11b was analyzed by flow cytometry and compared respectively.ROC curve of CD11b in 2 diabetes mellitus patients was made.Results The expression ratio of CD11b in high active CMV infection group and CMV infection group in type 2 diabetes mellitus patients was (41.25 ± 5.33)%.There was significant difference (P =0.019)compared with negative control group (61.13 ±5.28)% and there was significant difference (P =0.009)compared with negative control group (64.21 ±6.02)%;optimal cut -off value of CD11b in high active CMV infection group was 45.65%,the sensitivity was 100.00%,the specificity in high active CMV infection group was 60.12%,and the area under the receiver -operating curve(ROC)was 0.628;optimal cut -off value of CD11b in CMV infection group was 70.21%,the sensitivity of CD11b was 87.69%,the specificity of CD11b was 99.22%,and the area under of ROC of CD11b was 0.991.Conclusion The neutrophils CD11b expression in type 2 diabetes mellitus patients with CMV infection is down regulation.CD11b expression can be used as a laboratory diagnosis basis of CMV infection.
出处 《中国基层医药》 CAS 2016年第15期2264-2267,I0002,共5页 Chinese Journal of Primary Medicine and Pharmacy
关键词 2 型糖尿病 流式细胞术 CMV CD11b Type 2 diabetes mellitus Cytomegalovirus CD11b Flow cytometry
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