摘要
通过对竹席样品中的罗丹明B用乙醇水溶液提取，经C18反相色谱柱分离，荧光检测器进行测定。结果表明：罗丹明B在0.1～200μg／L浓度范围内线性相关系数为0.9999，在20、500、5000μg／kg添加水平时的回收率为80.6％～92.8％，相对标准偏差为1.3％～2.1％（n＝6），定量检出限为5.0μg／kg。高效液相色谱荧光检测法适用于竹席中罗丹明B的测定，并具有简单、快速、准确等优点。
Rhodamine B in samples were extracted with ethanol﹣water solution,separated on C18 reverse﹣phase chroma﹣tography column and detected by fluorescence detector. The results showed that,linear correlation coefficient was 0. 999 9,over the range of 0. 1 ~200 μg/L. The recoveries were 806% ~92. 8% for spiked standards at 20,500, 5 000 μg/kg levels with RSD of 1. 3%~2. 1% （n=6）,and the detection limit was 5. 0 μg/kg. The high performance liquid chromatography with fluorescence detection method has the advantages of simple,fast and accurate,is suitable for determination of rhodamine B in bamboo mat.
出处
《湖南林业科技》
2016年第4期54-60,共7页
Hunan Forestry Science & Technology
关键词
炭化竹席
罗丹明B
高效液相色谱
荧光检测
thermo﹣modified bamboo mat
rodamine B
HPLC
fluorescence detection
