期刊文献+

HPLC-MS同时测定药用紫草中13种紫草素和紫草呋喃类化合物的含量 被引量:8

Simultaneous Determination of 13 Shikonins and Shikonofurans in Medicinal Arnebia plants by HPLC-MS
原文传递
导出
摘要 目的 建立药用紫草中异戊酰紫草素,乙酰紫草素,β,β-二甲基丙烯酰紫草素,异丁酰紫草素,β-乙酰氧基-异戊酰紫草素,β-羟基异戊酰紫草素,丙酰紫草素,1-甲氧基乙酰紫草素,紫草素,紫草呋喃A,紫草呋喃B,紫草呋喃D和紫草呋喃E共13个紫草素与紫草呋喃类化合物的HPLC-MS含量测定方法。方法 在负离子电离条件下,采用MRM扫描模式,以0.1%甲酸水-10 mmol·L-1甲酸铵和乙腈为流动相,以Waters Sun Fire C18column(2.1 mm×150 mm,3.5μm)为色谱柱,在系统考察分析方法的线性、灵敏度、精密度、稳定性、重复性和加样回收率符合分析要求的基础上,对22批次市售紫草样品进行分析,准确评价紫草药材质量并分析其原因。结果 13个化合物在所选浓度范围内与峰面积呈良好线性关系,加样回收率为87.3%-107.6%,RSD值均小于9.0%。22批次市售紫草样品中的紫草素和紫草呋喃类成分存在较大差异。结论 该方法合理可行,优势明显,操作简便,可用于紫草及相关科属药用植物及其制剂的质量控制研究。 OBJECTIVE To establish a method for simuhaneous determination of 13 constituents including isovalerylshikonin, acetylshikonin, β, β-dimethylacrylshikonin, isobutyrylshikonin, β-acetoxy-isovaleryshikonin, β-hydroxyisovalel-ylshikonin, propio- nylshikonin, 1-methoxyacetylshikonin, shikonin, shikonofuran A, shikonofuran B, shikonofuran D, and shikonoturan E in Arnebia plants by HPLC-MS. METHODS The determination was performed on a Waters Sun Fire Cis colunm(2. 1 mmx150 ram,3.5 μm) with mobile phase A consisting of water containing 0. 1% formic acid and 10 mmol · L-1 ammonium formate and mobile phase B of acetonitrile under negative ionization MRM mode. The qualities of 22 batches of Arnebia plant.s samples obtained from medicinal materi- alsmarkets were investigated and evaluated after methodology validations. RESULTS There were good linear relationships between the peak areas and predetermined content ranges of these compounds. The average recoveries varied from 87.3% to 107.6%. The contents of shikonins and shikonofurans of the 22 batches of Arnebia plants samples showed significant difference. CON- CLUSION The established method is feasible and simple for the quality control of Medicinal Arnebia plants and their related plants and preparations.
作者 廖梅 姜宏梁
出处 《中国药学杂志》 CAS CSCD 北大核心 2016年第14期1212-1218,共7页 Chinese Pharmaceutical Journal
关键词 紫草属植物 液-质联用 紫草素 紫草呋喃 含量测定 Arnebia plants HPLC-MS shikonin shikonofuran content determination
  • 相关文献

参考文献20

  • 1LEEJ H,JUNG K M,BAE I H,et al.Anti-inflammatory and barrier protecting effect of Lithospermum erythrorhizon extracts in chronic oxazolone-induced murine atopic dermatitis[J].J Dermatol Sci,2009,56 (1):64-66.
  • 2ASHKANIESFAHANI S,IMANIEHM H,KHOSHNEVISZADEH M,et al.The healing effect of Arnebia euchroma in second degree burn wounds in rat as an animal model[J].Iran Red Crescent Me,2012,14 (2):70-74.
  • 3KAITH B S,KAITH N S,CHAUHAN N S.Anti-inflammatory effect of Arnebia euchroma root extracts in rats[J].J Ethnopharmacol,1996,55 (1):77-80.
  • 4Ch.P(2015).Vol Ⅰ(中国药典2015年版.一部)[S].2015:340.
  • 5詹志来,胡峻,刘谈,康利平,南铁贵,郭兰萍.紫草化学成分与药理活性研究进展[J].中国中药杂志,2015,40(21):4127-4135. 被引量:184
  • 6Qinghai Institute for Tibetan Medicines.Tibetan Drugs in China(中国藏药)[M].Vol 1.Shanghai:Shanghai Scientific and Technical Publishers,1996:347.
  • 7State Administration of Chinese Medicine.Chinese Materia Medica,Tibetan Drugs Edition(中华本草,藏药分册)[M].Shanghai:Shanghai Science and Technology Publishing House,2002:363.
  • 8李惠兰,秦剑,何洪静,张荣平,胡建林,赵荣华.滇紫草、新疆紫草和露蕊滇紫草提取物药效学研究[J].中国民族民间医药,2002,11(3):174-176. 被引量:17
  • 9LI F,LI Y H,LI H M,et al.Study on chemical constituents of Onosma paniculatum[J].云南中医中药杂志,2014,35(7):70-70.
  • 10CUI X Q.The latest research progress in chemical constituents and pharmacological effects of medicinal Zicao[J].济宁医学院学报,2015,38(5):356-358.

二级参考文献144

共引文献290

同被引文献70

引证文献8

二级引证文献46

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部