摘要
目的观察补脾益肾方对去卵巢骨质疏松症模型大鼠的影响,探讨其对围绝经期骨质疏松症的防治作用。方法将50只健康大鼠随机分为正常组、假手术组、模型组、中药组、西药组5组,每组10只。去卵巢手术制备骨质疏松症模型。药物治疗12周后,双能X线骨密度仪检测各组大鼠股骨骨密度,ELISA法检测血清ALP、OC、MMP-2、PICP、TGF-β1表达水平。结果与正常组比较,模型组骨密度水平显著下降(P<0.01),血清ALP、OC、MMP-2、PICP水平显著升高(P<0.01或P<0.05),TGF-β1水平显著降低(P<0.05);与模型组比较,中药组骨密度水平显著升高(P<0.01),血清ALP、OC、MMP-2、PICP水平显著下降(P<0.01或P<0.05),TGF-β1水平显著升高(P<0.05)。结论补脾益肾方对去卵巢骨质疏松症模型大鼠具有防治作用,能有效抑制骨量丢失,促进骨形成。
Objective To observe the effect of Invigorating Spleen and Tonifying Kidney Prescription on ovariectomzed osteoporotic rats,and to discuss the preventive and treatment effect on postmenopausal osteoporosis. Methods The 50 rats were divided randomly into Normal Group,Shan operation Group,Model Group,Chinese herb Group,Western medicine Group. Ovariectomy was performed to create the rat osteoporosis model. After 12 weeks treatment,BMD of femur was detected using dual energy X-ray bone densitometry. ALP,OC,MMP-2,PICP,TGF-β1 expression were determined using by ELISA. Results Compared with Normal Group,Model Group has significantly increased（ P〈 0. 01） in ALP,OC,MMP-2,PICP levels（ P〈 0. 01,P〈 0. 05）,decreased in TGF-β1 level and BMD of femur（ P〈 0. 01,P〈 0. 05）,Compared with Model Group,Chinese herb Group has significantly increased in TGF-β1 level and BMD of femur（ P〈 0. 01,P〈 0. 05）,decreased in ALP,OC,MMP-2,PICP levels（ P〈 0. 01,P〈 0. 05）,TGF-β1were significantly increased（ P〈 0. 05）. Conclusion Invigorating Spleen and Tonifying Kidney Prescription has the preventive and treatment effect on osteoporosis and ovariectomzed rats,It can effectively inhibit the bone loss,promote bone formation.
出处
《吉林中医药》
2016年第7期709-712,共4页
Jilin Journal of Chinese Medicine
基金
国家自然科学基金项目(81001493)
关键词
围绝经期骨质疏松症
去卵巢大鼠
补脾
益肾
peri menopausal osteoporosis
ovariectomized rats
tonifying spleen
invigorate kidney
