摘要
为明确番鸭细小病毒结构蛋白（VP1）基因的分子特征,本研究利用PCR方法从番鸭细小病毒（MDPV）黑龙江分离株YL08中扩增出VP1基因,并对其进行了克隆测序和分析。基因测序结果表明：MDPV YL08株VP1基因全长为2 199 bp,编码732个氨基酸;MDPV YL08株VP1基因与国内外其他已发表的MDPV分离株核苷酸序列的同源性为92.7%~95.8%;系统进化树分析表明：MDPV各分离株在遗传进化上存在较为明显的地域性。本研究中MDPV YL08株与其他MDPV中国大陆分离株处于不同的分支,提示其可能具有独特的遗传起源。
In order to elucidate the molecular characteristics of muscovy duck parvovirus（ MDPV） structural protein 1（ VP1）,the target VP1 gene was amplified from MDPV YL08 strain by PCR with specific primers,and then the PCR product was cloned in to the vector pET-32a（ ＋） and sequenced. The sequencing result showed that the VP1 gene consisted of 2199 nucleotides and encoded 732 amino acids. The VP1 gene of MDPV YL08 strain shared 92. 7% ~ 95. 8% nucleotide homology with those of other published MDPV isolates. The phylogenetic tree showed that there are obvious regional characteristics in the genetic evolution of MDPV isolates. MDPV YL08 strain was in the different branch from other MDPV isolates in the Mainland of China,indicating that MDPV YL08 strain had a unique genetic origin.
出处
《畜牧与兽医》
北大核心
2016年第7期40-43,共4页
Animal Husbandry & Veterinary Medicine
基金
黑龙江省自然科学基金(QC2014C034)
黑龙江省普通本科高等学校青年创新人才培养计划(UNPYSCT-2015093)
齐齐哈尔大学青年教师科研启动项目(2014k-M06
2014k-Z02)
关键词
番鸭细小病毒
VP1
克隆
序列分析
muscovy duck parvovirus
VP1
cloning
sequence analysis
