摘要
以广西大学果树育种基地的番荔枝为试材,对非洲骄傲和凤梨释迦的花粉进行电镜扫描;自交和正反交授粉,调查着果率,荧光显微压片观察花粉萌发及生长动态。结果表明,非洲骄傲和凤梨释迦的花粉均为辐射对称型,外壁均为穴状纹孔,形状较为相似;赤道面观近椭圆形,极面观近圆形。两个番荔枝品种自交和杂交后花粉在柱头上都能萌发,但品种间存在萌发速度的差异。授粉后1h,花粉管都开始萌发,但数量有差异,凤梨释迦自交和杂交已经大量萌发,而非洲骄傲自交和杂交的则在14h才大量萌发。授粉24h,4个处理的花粉管均进入子房。凤梨释迦处理在48h后达到胚囊。这两个番荔枝品种的花粉形态相似,自交和杂交均有较好的亲和性。
This study was to observe morphology of pollen under SEM and the pollination compatibility of two cultivars of atemoya(Annona cherimoya × A.squamosa),African Pride and Fenglishijia,in order to provide a scientific basis to the cultivation and hybrid breeding of atemoya.Morphology of pollen under SEM and growth of pollen tubes in the styles of atemoya and the fruit setting after self-and cross-pollination was examined.Results showed that African Pride and Fenglishijia exhibited similar pollen morphology.For both cultivars,the shape of pollen was actinomorphy,and the ornamentation of exine was cave-shaped.African Pride presented an oval shape in equatorial view and round in polar view,similar to those of Fenglishijia.Pollens of two cultivars germinated from both self-and cross-pollination.However,the timing of pollen germination between African Pride and Fenglishijia was different.Large number of pollen tubes presented in Fenglishjia at 1hafter pollination,but 14 hafter pollination in African Pride.The pollen tubes of all self-and cross-pollination were observed to reach the ovary 24 hafter pollination.The pollen tubes reached ovule in Fenglishijia 48 hafter pollination.We proposed that African Pride and Fenglishijia have similar pollen morphology with good compatibility under self-and cross-pollination.
出处
《中国南方果树》
北大核心
2016年第4期18-22,共5页
South China Fruits
基金
东盟热带亚热带特色果树品种资源引进选育研究(桂科合14123001-1-3)资助
关键词
番荔枝
花粉形态
授粉亲和性
荧光显微观察
Annona atemoya
pollen morphology
pollination compatibility
fluorescence microscopy