摘要
目的:研究IL-17对病毒性心肌炎小鼠的作用。方法:随机挑选20只野生型BALB/c小鼠为对照组,20只IL-17A^(- /-)小鼠为IL-17A^(- /-)组,20只L-17A^(- /-)小鼠为IL-17组。每只小鼠腹腔注射柯萨奇病毒B3(Coxsackie virus B3,CVB3)建立VMC模型。收集小鼠外周血利用ELISA法检测血清中IL-17水平,利用流式细胞术检测血清中Th17细胞水平。CVB3处理3 d后,对照组和L-17A^(- /-)组腹腔注射100μg Ig G抗体,IL-17组注射100μg IL-17m Ab。分别于CVB3处理第3、7、14天收集小鼠心肌组织。将小鼠心肌切片并H&E染色进行病理学检查。检测小鼠心肌组织中病毒滴度。利用酶联免疫吸附法检测心肌组织IL-17、IL-23、IL-6和TNF-α的含量。结果:成功构建病毒性心肌炎小鼠。对第14天收集的组织进行分析发现,IL-17组小鼠血清中IL-17和Th17水平均显著低于对照组和IL-17^(- /-)组。对照组小鼠心肌组织损伤程度明显高于IL-17A^(- /-)组和IL-17组,IL-17小鼠心肌组织损伤程度高于IL-17A^(- /-)组。对照组病毒滴度高于IL-17A^(- /-)组和IL-17组,且随着CVB3处理时间增加而增加。在补充IL-17抗体后IL-17组病毒滴度高于IL-17A^(- /-)组。在IL-17^(- /-)组和IL-17组的IL-17、IL-23、IL-6和TNF-α水平均显著低于对照组。在补充IL-17抗体后IL-17组IL-17、IL-23、IL-6和TNF-α水平均显著高于IL-17^(- /-)组(P<0.05)。结论:IL-17是参与病毒性心肌炎的重要炎症因子,而IL-17缺失可保护小鼠心肌免受病毒性心肌炎损伤。
Objective： To study the effect of IL-17 on viral myocarditis in mice. Methods： We randomly selected 20 wild type BALB / c mice as the control group,20 IL-17A^- /-mice for IL-17A^- /-group,20 IL-17A^- /-mice for IL-17 group. Each mouse had intraperitoneal injection of Coxsackie virus B3（ Coxsackie virus B3,CVB3） to construct VMC model. The serum levels of IL-17 were detected by ELISA method,and Th17 cells were detected by flow cytometry. After 3 days of treatment with CVB3,100 μg Ig G antibody was injected intraperitoneally in control group and L-17A^- /-group,and 100 μg IL-17 m Ab was injected with IL-17 group. Mice myocardium was collected at 3rd,7th and 14 th days,respectively,after CVB3 treatment. Pathological examination was carried out on the myocardial sections of mice and stained with HE. Virus titer in mouse myocardium was examined. The contents of TNF-α,IL-6,IL-23 and IL-17 in myocardial tissue were detected by enzyme linked immunosorbent assay. Results： We successfully constructed the model of mice with viral myocarditis. The levels of Th17 and IL-17 in serum of IL-17 group were significantly lower than those in control group and IL-17^- /-group. On the fourteenth day after CVB3 treatment,the degree of myocardial tissue damage in the control group was significantly higher than that in the IL-17A^- /-group and the IL-17 group（ P〈0. 05）,the degree of myocardial injury in IL-17 mice was higher than that in the IL-17A^- /-group. The virus titer of the control group was higher than that of IL-17A^- /-group and IL-17 group,and the control group was increased with the increase of CVB3 treatment phase（ P〈0. 05）. After the IL-17 m Ab was injected,the virus titer of IL-17 group was higher than that of IL-17A^- /-group. The levels of IL-17,IL-23,IL-6 and TNF-α in IL-17^- /-group and IL-17 group were significantly lower than those in control group. The levels of IL-17,IL-23,IL-6 and TNF-α in IL-17 group were significantly higher than those in IL-17^- /-group（ P〈0. 05）. Conclusion： IL-17 is an important inflammatory factor in viral myocarditis,and IL-17 deletion can protect myocardium from viral myocarditis in mice.
出处
《中国免疫学杂志》
CAS
CSCD
北大核心
2016年第7期979-982,共4页
Chinese Journal of Immunology
