摘要
目的:探讨下调microRNA-214(miR-214)表达对人卵巢癌SKOV-3细胞迁移和侵袭的作用及其可能机制。方法:采用脂质体介导法将miR-214抑制剂转染人卵巢癌SKOV-3细胞,Real-time PCR法检测miR-214、核因子-κB(nuclear factor-κB,NF-κB)和尿激酶型纤溶酶原激活剂(uridylyl phosphate adenosine,u PA)基因mRNA的表达,Western blotting法检测细胞NF-κB和u PA蛋白表达。划痕试验检测细胞迁移能力,Transwell小室法检测细胞侵袭能力。结果:经转染miR-214抑制剂后,人卵巢癌SKOV-3细胞miR-214表达降低,细胞迁移和侵袭能力降低。同时NF-κB和u PA mRNA和蛋白表达也降低。结论:下调人卵巢癌SKOV-3细胞miR-214表达可抑制细胞迁移和侵袭能力,下凋NF-κB和u PA基因表达可能是其作用机制。
Objective： To observe effect of down-regulating microRNA-214（ miR-214） expression on migration and invasion of ovarian carcinoma SKOV-3 cells and explore its possible mechanism. Methods： miR-214 inhibitors were transfected into human ovarian carcinoma SKOV-3 cells by liposome. Expressions of miR-214,nuclear factor-κB（ NF-κB） and urokinase-type plasminogen activator（ uridylyl phosphate adenosine [u PA]） gene mRNA,as well as NF-κB and u PA proteins in the cells were respectively detected by Real-time PCR and Western blotting assays. Scarification test and transwell assay were used to detect abilities of migration and invasion of the cells respectively. Results： After transfection of miR-214 inhibitor,expression of miR-214 in human carcinoma SKOV-3 cells decreased,and migration and invasion abilities of the cells reduced. At the same time,expressions of NF-κB and u PA gene mRNA and proteins also depressed. Conclusion： Down-regulation of miR-214 expression in human ovarian carcinoma SKOV-3 cells could inhibit migration and invasion abilities of the cells,and down-regulation of NF-κB and u PA gene expressions might be its possible mechanism.
出处
《中国肿瘤生物治疗杂志》
CAS
CSCD
北大核心
2016年第4期506-509,共4页
Chinese Journal of Cancer Biotherapy
基金
武警后勤学院创新团队课题资助(No.whtd2013-2)~~
