摘要
【目的】温度适应性是决定昆虫分布和扩散的重要因素。为探索茶淡黄刺蛾Darna trima(Moore)对温度胁迫的耐受性,本研究测定了温度胁迫对4种抗氧化酶的活力和总抗氧化能力的影响。【方法】分别将茶淡黄刺蛾老熟幼虫置于﹣5、0、5、26(对照)、35、37.5、40℃下处理2、4、6 h,测定其体内超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、谷胱甘肽巯基转移酶(GST)和总抗氧化能力(T-AOC)的活力变化。【结果】茶淡黄刺蛾的4种抗氧化酶的活力和总抗氧化能力在不同温度和时间之间均存在显著变化,但不同指标对温度胁迫具有不同的响应模式。低温胁迫尤其是﹣5℃对各指标影响最为显著,而高温胁迫影响较小。【结论】SOD、CAT、POD、GST和T-AOC在茶淡黄刺蛾应对温度胁迫中具有重要作用,本研究可为判断茶淡黄刺蛾在茶区的分布范围提供一定的理论依据。
[Objectives] Temperature adaptability is an important factor affecting the distribution and geographic spread of insects. To explore resistance to temperature stress in Darna trima（Moore）, we investigated the activity of four protective enzymes and overall antioxidant capacity in this insect. [Methods] Mature larvae of D. trima were exposed to ﹣5, 0, 5, 26（control）, 35, 37.5 and 40 ℃for 2, 4 and 6 h, and the activities of superoxide dismutase（SOD）, catalase（CAT）, peroxidase（POD）, glutathione S-transferase（GST）, and total antioxidant capacity（T-AOC）, were measured. [Results] Different temperatures and exposure durations had significant effects on the activities of the four antioxidant enzymes and overall antioxidant capacity, and produced different response patterns. Cold stress, especially ﹣5℃, had significant effects on all indices, however, heat stress had relatively small effects. [Conclusion] SOD, CAT, POD, GST and T-AOC may play important roles in the response of D. trima to temperature stress. These results provide a theoretical basis for estimating the distribution of D. trima in tea plantations.
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2016年第4期809-816,共8页
Chinese Journal of Applied Entomology
基金
国家茶叶产业技术体系西部病虫害防控岗位专家基金(CARS-23)
关键词
茶淡黄刺蛾
温度胁迫
保护酶
总抗氧化能力
Darna trima
temperature stress
protective enzyme
total antioxidant capacity
