摘要
利用SCoT分子标记对47个菌草种质资源进行遗传多样性分析。从20个SCoT引物中筛选出10个扩增效果好的引物,对47份菌草种质材料的DNA进行PCR扩增,共获得282个多态片段。结合UPGMA聚类分析方法,算出47份种质材料间的遗传相似系数(SM)在0.77-0.96,所有47份材料被区分开来,在遗传相似系数为0.815时,47份材料可分为5大类群。研究结果表明,SCoT分子标记技术可有效用于菌草资源的遗传多样性分析。
The genetic diversity of 47 Juncao germplasms was analyzed by SCoT markers. Twenty ScoT primers were first used for screening on a subset of four germplasms, of which 10 gave good amplification patterns and were then used for analyzing the DNA of 47 JUNCAO germplasms. A to- tal of 282 polymorphic DNA fragments were scored among the 47 JUNCAO germplasms from the electrophoresis patterns of the 10 selected SCoT primers. By using the NTSYS-pc 2.1 software com- bined with UPGMA clustering analysis method, the genetic similarity coefficient （SM） was calculated among all accessions and ranged from 0.77 to 0.96. All the 47 accessions were distinguished from each other. The 47 JUNCAO germplasms were clustered into five groups when the genetic similarity coefficient was 0.815. Our results showed that the SCoT markers could be effectively used for anal- ysis of genetic diversity of Juncao germplasms.
出处
《热带农业工程》
2016年第2期29-34,共6页
Tropical Agricultural Engineering
基金
国家菌草工程技术研究中心攻关课题项目(No.JCGG14010)
