期刊文献+

南方红豆杉水提物对HER2阳性人NCI-N87胃癌移植瘤生长及凋亡作用 被引量:10

Taxus Chinensis Var. Mairei( AETC) Inhibiting HER2 Positive Human Gastric Cancer Cells NCI-N87 Transplantation Tumor and Inducing Apoptosis
原文传递
导出
摘要 目的:探讨南方红豆杉水提物对HER2阳性人胃癌NCI-N87细胞裸鼠移植瘤的抑制作用,及诱导凋亡的作用。方法:通过建立人胃癌NCI-N87细胞裸鼠移植瘤模型,采用随机数字法将80只裸鼠随机分为8组:生理盐水对照组,南方红豆杉低、中、高剂量组,西药赫赛汀组,南方红豆杉低、中、高剂量联合赫赛汀组;给药处理后颈椎脱臼法处死裸鼠,剥离瘤组织,称瘤重、测量瘤径,计算移植瘤体积,绘制肿瘤生长曲线,计算瘤重得抑瘤率;TUNEL法检测移植瘤细胞凋亡,计算凋亡指数。结果:同对照组比较治疗组均可抑制肿瘤生长(P<0.05),且联合组表现出比单药组更为显著的抑制作用;中剂量联合组对肿瘤的抑制作用优于红豆杉高剂量及赫赛汀单药组(P<0.05)。对照组凋亡细胞数少,凋亡率低,低、中、高剂量单药组均可见到凋亡细胞,凋亡率同对照组比较(P<0.05);各剂量联合用药组凋亡细胞数目较多,与单药组比较均有明显差异(P<0.05),中剂量联合组差异性最为显著(P<0.01)。结论:南方红豆杉水提物对HER2阳性高表达NCI-N87胃癌裸鼠移植瘤的生长有抑制作用,联合赫赛汀可以增强其增殖抑制效果,中剂量联合组显著提高赫赛汀的抑瘤作用;南方红豆杉水提物可以促进胃癌细胞的凋亡,联合赫赛汀能增强其诱导凋亡作用。 Objective: It was to investigate the effect of AETC on growth of human gastric cancer cells NCI-N87 subcutaneously transplanted in nude mice and the action of inducing apoptosis. Methods: Eighty BALB / C nude mice subcutaneously transplanted with NCI-N87 cells were randomly divided into eight groups( 10 mice per group) : NS group,low,medium and high dose AETC groups,Herceptin group,low,medium and high dose AETC combined with Herceptin groups. Tumor volume and size were observed and measured every 3 days. Give the corresponding drugs when the tumor formation by lavage every day. Mice were killed by cervical dislocation method. Measure the tumor size,volume and weight immediately and draw the growth curve and calculate the rate of tumor inhibition after peeling off the tumor. Apoptosis would be detected with TUNEL transplanted tumor cells after calculating the apoptosis index. Results: The results showed that all the treatment groups can inhibit tumor growth when compared with the control group( P 〈 0. 05) and more significant than only AETC. Medium dose of AETC combined with Herceptin group had the higher suppression function than high dose of AETC group and Herceptin group( P 〈 0. 05). A few apoptosis cells in control group,low,medium and high dose AETC groups’ cells were visible compared with the control group’s( P 〈 0. 05),presenting a certain dose-response effect. Combined groups had more apoptosis cells and significant difference compared with the single AETC groups or Herceptin group( P 〈 0. 05). Medium dose of AETC group was more significant( P 〈 0. 01). Conclusion: These findings indicated that the treatment groups can inhibit the growth of transplanted tumor and combining Herceptin can enhance the proliferation inhibition effect of single drug groups. Medium dose of AETC can enhance the proliferation inhibition effect of Herceptin significantly. AETC can promote the apoptosis of gastric carcinoma and the combined groups can play a better role to induce apoptosis.
出处 《中华中医药学刊》 CAS 北大核心 2016年第8期1949-1952,I0009,共5页 Chinese Archives of Traditional Chinese Medicine
基金 浙江省自然科学基金项目(LY13H290013) 浙江省中医药科技计划项目(2013ZA043) 浙江省重中之重学科"中西医结合"项目
关键词 南方红豆杉水提物 人NCI-N87胃癌细胞 移植瘤 凋亡 AETC human NCI-N87 gastric cancer cells transplantation tumor apoptosis
  • 相关文献

参考文献15

  • 1Bernard Stewar.world canner report 2014[C].The International Agency for Research on Cancer,2014.
  • 2陈万青,张思维,曾红梅,郑荣寿,邹小农,赵平,吴良有,李光琳,赫捷.中国2010年恶性肿瘤发病与死亡[J].中国肿瘤,2014,23(1):1-10. 被引量:882
  • 3Hofmann M,Stoss O,Shi D,et al.Assessment of a HER2 scoring system for gastric cancer:results from a validation study[J].Histopathology,2008,52:797-805.
  • 4Bang YJ,Van Cutsem E,Feyereislova A,et al.Trastuzumab in combination with chemotherapy versus chemotherapy alone for treatment of HER2-positive advanced gastric or gastro-oesophageal junction cancer(To GA):a phase3,open-label,randomised controlled trial[J].Lancet,2010,376:687-697.
  • 5Wainberg,ZA,Anghel,A,Desai,AJ,et al.Lapatinib,a dual EGFR and HER2 kinase inhibitor,selectively inhibits HER2-amplified human gastric cancer cells and is synergistic with trastuzumab in vitro and in vivo[J].Clin Cancer Res,2010,16(5):1509-1519.
  • 6Kim ES,Salgia R.MET pathway as a therapeutic target[J].J Thorac Oncol,2009,4(4):444-447.
  • 7张晶,舒琦瑾,高继良,张乐.南方红豆杉水提物对肿瘤细胞增殖抑制作用的研究[J].中国中西医结合杂志,2013,33(6):805-809. 被引量:16
  • 8刘静芳,汤在祥,胡建铭.赫赛汀联合顺铂对人卵巢癌SKOV3裸小鼠移植瘤的抑制作用研究[J].吉林医学,2013,34(22):4421-4423. 被引量:5
  • 9Ling Y,Lu N,Gao Y,et al.Endostar induces apoptotic effects in HUVECs through activation of caspase-3 and decrease of bcl-2[J].Anticancer Res,2009,29(1):411-417.
  • 10温少瑾,郝敬全,江劲波.中药诱导肿瘤细胞凋亡机制的研究进展[J].湖南中医杂志,2013,29(5):125-127. 被引量:5

二级参考文献88

共引文献928

同被引文献127

引证文献10

二级引证文献14

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部