摘要
目的:在H2O2胁迫下,研究刺老苞总皂苷增强MC3T3-E1成骨细胞抗自由基损伤的作用。方法:用H2O2作用MC3T3-E1成骨细胞,建立自由基损伤细胞模型。培养的成骨细胞分为对照组、模型组、刺老苞总皂苷低剂量组(1×10-8mol/L)、刺老苞总皂苷中剂量组(1×10-7mol/L)和刺老苞总皂苷高剂量组(1×10-6mol/L)。测定不同处理组细胞活力、超氧化物歧化酶(superoxide dismutase,SOD)活性、活性自由氧(reactive oxygen species,ROS)含量、脂质过氧化物(lipid oxygen,LPO)含量,同时利用荧光偏振法测定细胞膜流动性。结果:模型组细胞活力、SOD活性、细胞膜流动性均比不同刺老苞总皂苷组有显著降低(P﹤0.01),而ROS含量、LPO含量均比不同刺老苞总皂苷组有显著升高(P﹤0.01)。结论:H2O2摄入会导致MC3T3-E1成骨细胞的氧化损伤,而刺老苞总皂苷可以预防或降低此类损伤对细胞的影响。
Objective ： To investigate the effect of Aralia echinocauIis Hand. Mazz total saponin on enhances the function of anti - oxidative damage in MC3T3 - E1 osteoblast. Methods ： MC3T3 - E1 osteoblasts were randomly divided in five groups, including control group, model group, Aralia echinocauIis Hand. Mazz total saponin group [low dose （1 × 10-Smol/L）,middle dose （1× 10-Tmol/L）,high dose （1× 10-6mol/L）], whose activity of cells, superoxide dismutase （ SOD ）, the content of reactive oxygen species （ ROS ）, lipid oxygen （LPO） and membrane fluidity were tested and compared. Results： Compared with Aralia echinocauIis Hand. Mazz total saponin groups, the activity of cells, SOD activity and membrane fluidity in the model group were significantly decreased （ P 〈 0.01 ）. However, the content of ROS and LPO were sion： H2O2 can cause oxidative damage of MC3T3 - E1 osteoblasts, aponin can prevent and decrease its influence significantly raised（ P 〈 0.01 ）. Conclubut Aralia echinocauIis Hand. Mazz total
出处
《中国民族医药杂志》
2016年第6期43-46,共4页
Journal of Medicine and Pharmacy of Chinese Minorities
基金
国家自然科学基金(81173361
81270051
81473451)
