摘要
探究了超声波辅助提取夏黑葡萄花色苷的最佳工艺及其抗氧化能力。采用Box-Behnken响应面法研究液料比、超声时间和温度这3个因素及其交互作用对花色苷提取的影响。结果表明,优化后的提取工艺为:液料比为3∶1(m L/g),超声时间为21 min,温度为59℃。此条件下提取的总花色苷含量和模型预测值的相对误差仅为3.17%。还考察了夏黑葡萄花色苷对羟基自由基(·OH)、超氧阴离子自由基(·O_2^-)、DPPH自由基(DPPH·)的清除能力,研究结果表明,本实验提取的花色苷具有较强的抗氧化活性,其清除自由基的能力与浓度存在量效关系。
The optimized experimental condition of anthocyanin from summer black grape by ultrasonic-assisted extraction as well as its antioxidant properties were studied. Box-Behnken response surface design method was employed to explore the effect of liquid-solid ration,ultrasonic time and temperature on anthocyanin-extraction. The optimal conditions obtained were the liquid-solid ration of 3 ∶ 1,ultrasonic time of 21 min and temperature of 59 ℃. Under the optimized experimental conditions,relative error of total anthocyanin content as-extracted was 3. 17% compared with theoretical prediction value. Furthermore,scavenging activity towards ·OH,·O2- and DPPH · caused by anthocyanin were also systematically investigated,respectively. These results demonstrated that the anthocyanin as-extracted displayed strong antioxidant activity,and its scavenging activity was evaluated by the concentration of anthocyanin.
出处
《应用化工》
CAS
CSCD
北大核心
2016年第8期1418-1423,共6页
Applied Chemical Industry
基金
国家科技支撑计划(2014BAD07B02)
酿酒生物技术及应用四川省重点实验室项目(NJ2014-03)
重庆大学大型仪器设备开放基金
关键词
夏黑葡萄
花色苷
响应面法
抗氧化能力
summer black grape
anthocyanins
response surface method
antioxidant activity
