摘要
目的应用meta分析进一步观察CYP1A1基因多态性对接触多环芳烃(polycyclic aromatic hydrocarbons,PAHs)人群尿液中1-羟基芘(1-hydroxypyrene,1-OHP)和外周血DNA加合物水平的影响。方法检索PubMed、Web of Science、中国知网和万方数据库,文献出版时间截止到2015年8月。中文检索词为多环芳烃、CYP1A1、多态性、1-羟基芘和DNA加合物及其组合,英文检索词为PAH或polycyclic aromatic hydrocarbon、CYP1A1、polymorphism、1-OHP或1-hydroxypyrene、DNA加合物及其组合。按照纳入和排除标准选择文献、提取资料后,应用Review Manager 5.3和STATA 11.0软件进行meta分析。结果纳入meta分析的1-羟基芘和DNA加合物的文献分别为12、4篇,结果显示,所有暴露组、职业暴露组和非职业暴露组CYP1A1基因rs4646903位点T/C基因型或C/C携带者1-OHP水平均高于T/T携带者,合并效应量WMD及其95%CI分别为0.05(0.02,0.07)、0.08(0.04,0.12)和0.03(0.00,0.06)。CYP1A1基因rs1048943位点Ile/Val或Val/Val携带者与Ile/Ile携带者间1-OHP水平差异无统计学意义(P>0.05),CYP1A1基因rs4646903及rs1048943位点多态性与DNA加合物关联均无统计学意义(P>0.05)。结论 CYP1A1基因rs4646903位点携带C基因能够显著促进PAHs的代谢。
Objective To assess the influence of CYP1A 1 polymorphism on the concentration of urinary 1-hydroxypyrene （1- OHP） and DNA adduct level in general population or occupationally polyeyclic aromatic hydrocarbons （PAHs）-exposed workers by a meta-analysis. Methods PubMed, Web of Science, CNKI, and Wan Fang Database were used and the original papers published before August 2015 were included. PAHs （or polycyclic aromatic hydrocarbons）,CYP1Al,polymorphism, 1-OHP （or 1-hydroxypyrene） and DNA adducts were used as the key words. According to the inclusion and exclusion criteria for screening studies,pooled estimates were summarized by RevMan 5.3 and STATA 11.0 software. Results Twelve and four eligible studies were included in the meta-analysis for 1-OHP and DNA adduet, respectively. The results showed that CYP1A 1 rs4646903 T/C or C/C carriers in all study groups, occupational workers and non-occupational group had a significant higher 1-OHP level than T/T carriers,the combined weighted mean differences （95% C/） were 0.05 （0.02-0.07）,0.08 （0.04-0.12） and 0.03（0.00-0.06）, respectively. No significant difference in 1-OHP was found between CYP1A 1 rs1048943 Ile/Val, or Val/Val carriers and Ile/Ile carriers（P〉0.05）. No association was found for both CYP1A 1 rs4646903 and rs1048943 between their polymorphism and DNA adduet level（P〉0.05）. Conclusion CYP1A 1 rs4646903 C carrier seems to contribute significantly to the metabolism of PAHs.
出处
《环境与健康杂志》
CAS
北大核心
2016年第6期525-531,共7页
Journal of Environment and Health
基金
国家自然科学基金(81372955)
青岛市医疗卫生优秀人才培养项目
