摘要
为获得自然感染小熊猫的犬细小病毒(CPV)野毒株,以快速检测试纸确诊的且具有犬细小病毒病临床症状的发病死亡小熊猫肛门棉拭子、口腔棉拭子和抗凝血为病料进行CPV的PCR检测后,将病料预处理并接种于F81细胞盲传至培养细胞出现病变,收集细胞毒并进行PCR鉴定,接种细胞毒给2月龄健康幼犬进行回归试验。结果显示,小熊猫肛门棉拭子、口腔棉拭子和抗凝血的CPV PCR检测均为阳性。病料接种F81细胞盲传第5代时,细胞病变明显,细胞接毒72h后出现拉网、皱缩、崩解、脱落。细胞毒的PCR扩增产物测序结果与已经发表的CPV-js23和2010-BJ-A64毒株相应序列的同源性均为100%。接种细胞毒的幼犬出现了犬细小病毒病特征性症状。结果表明,获得了对小熊猫毒力较强的CPV野毒株。
To isolate and identify a CPV strain from a captive red panda,blood samples and rectal and saliva swabs were collected from the red panda with typic symptoms of canine parvovirus disease.After pretreatment,samples were detected by PCR,and inoculated in F81 cells.The F81 cell cultures with CPE were collected and identified by PCR, and used to challenage yang dogs. The results showed that, blood samples, rectal and saliva swabs were CPV positive,and CPE appeared in third F81 cell blind passage,and the PCR products were sequenced and identified as CPV. The challenaged dogs showed typic symptoms of canine parvovirus disease.The results indicated that a CPV strain,which may cause canine parvovirus disease in red pandas,was isolated successufly.
出处
《动物医学进展》
北大核心
2016年第8期124-127,共4页
Progress In Veterinary Medicine
关键词
犬细小病毒
小熊猫
病原分离
Canine parvovirus
red panda
pathogen isolation
