摘要
从野生蓝莓中提取总花青素,并采用XAD-7大孔树脂进行纯化,用超氧阴离子和双氧水自由基清除进行体外抗氧化、小白鼠耳肿足肿模型进行抗炎、Alamar Blue法进行体外抗肿瘤等生物活性评价。结果表明:野生蓝莓花青素抗氧化能力显著,与浓度梯度呈正相关关系;在浓度800μg/m L时对超氧阴离子的清除率达到86.86%,浓度为100μg/m L时对双氧水自由基的清除率达到73.93%;并且具有良好的抗炎能力,在100μg/g剂量灌胃时,对耳廓肿胀抑制率为47.04%,足肿抑制在第4小时达到最大作用;体外抗肿瘤活性也十分显著,对白血病细胞K562增殖抑制的IC50为79.01μg/m L,以上结果表明野生蓝莓具有多种生物活性,为其深入研究和开发利用提供了理论依据。
Anthocyanins was extracted from wild blueberry. The evaluations were made on activities of antioxidant in vitro with superoxide anion and hydrogen peroxide radical scavenging,mice in vivo anti-inflammatory with mice swollen foot swollen ear inflammatory model and anti-tumor activity in vitro by Alamar Blue method after purified by XAD-7 macroporous resin. Results： the anthocyanins of Chinese wild blueberry have obvious antioxidant capacity,and have a positive correlation between the concentration; in concentration of 800 μg / m L,the superoxide anion scavenging rate was 86. 86%,and at a concentration of 100 μg / m L,the hydrogen peroxide scavenging rate come to 73. 93%; in addition,the anthocyanins have good anti-inflammatory capabilities,at 100 μg / g oral dose,inhibition of ear swelling was 47. 04%,which foot swelling inhibition in the first four hours reach a maximum; moreover,their antitumor activity is also very significant,the inhibition of K562 leukemia cell proliferation IC50 was 79. 01 μg / m L. The results above show that Chinese wild blueberry has a variety of biological activity,which offer the foundation in their indepth study of the development and utilization.
出处
《食品与机械》
CSCD
北大核心
2016年第7期132-135,195,共5页
Food and Machinery
基金
上海市2015高校内涵建设项目(编号:A1-2018-15-0009)
关键词
野生蓝莓
总花青素
抗氧化
抗炎
抗肿瘤
wild blueberries
anthocyanins
antioxidant
anti-inflammatory
anti-tumor
