摘要
本研究结合温室盆栽和大田试验,研究了接种不同根瘤菌菌株对甘农3号(Medicago sativa cv.Gannong No.3)和陇东苜蓿(M.sativacv.Longdong)幼苗生长及结瘤特性的影响,并进一步分析了接种根瘤菌后陇东苜蓿的光合特性及其生物量构成因素的变化。结果表明,盆栽条件下,甘农3号接种17767菌株后,生物量、株高、分枝数和叶数分别增长了102%、100%、17%和18%;陇东苜蓿接种17625菌株后,生物量、株高、分枝数和叶数分别增了187%、48%、80%和47%。而在大田条件下,甘农3号接种17650菌株后,生物量和株高分别增长了21%和13%;陇东苜蓿接种17574菌株后,生物量和株高分别增长了348%和70%。盆栽条件下,相比未接种处理,接种6-3、17537、129、17670、17582、17578、17650菌株均不同程度地提高了陇东苜蓿的水分利用效率,仅接种17578、17582菌株提高了其光合速率。综合盆栽与田间试验,与甘农3号最佳共生匹配的根瘤菌菌株为B2;而与陇东苜蓿最佳共生匹配的根瘤菌菌株为17650。
Using a combination of greenhouse pot and field experiments, this study determined the effect of inoculation with different rhizobial strains on seedling growth and nodulation capacity of two cultivars of Medicago sativa. Further, seedling photosynthetic characteristics and biomass components of M. sativa cv. I.ongdong were analyzed. The results showed that seedling biomass, plant height, number of branches and number of leaves of M. sativa cv. Gannong No.3 increased by 102%, 100%, 17% and 102%, respectively, after inoculation with 17767 strains in greenhouse; while Longdong inoculated with 17625 strains increased seedling biomass, plant height, number of branches and number of leaves by 187%, 48%, 80% and 47%, respectively. In the field condition, seedling biomass and plant height of M. sativa cv. Gannong No.3 was increased by 21 % and 13%, respectively after inoculation with 17650 strains; while Longdong cultivar inoculated 17574 strains increased seedling biomass and plant height by 348% and 70%, respectively. Inoculated 6- 3, 17537, 129, 17670, 17582, 17578 and 17650 strains treatments increased plant water use efficiency to some extent in LongBased on their performance in the greenhouse and field condition, M, ativa cv, Gannong No.3 and M. sativa cv. Longdong was B2
出处
《草业科学》
CAS
CSCD
北大核心
2016年第8期1536-1549,共14页
Pratacultural Science
基金
公益性行业(农业)科研专项(201403048-3)
甘肃省重大科技攻关项目(2013GS 05907)
关键词
甘农3号
陇东苜蓿
根瘤菌
光合速率
水分利用效率
Medlcago sativa cv. Gannong No.3
Medicago sativa water use efficiency only by inoculating 17578 and 17582 strains. the optimal symbiotic rhizobial strains with and 17650, respectively. cv. Longdong
rhizobium
photosynthesis