摘要
目的研究化学发光微粒子免疫分析(CMIA)在丙型肝炎诊断中的应用价值。方法收集2014年4月至2015年4月该院住院患者350例血清标本,依照临床诊断分为丙型肝炎病例组(128例)和非丙型肝炎对照组(222例),分别用酶联免疫吸附试验(ELISA)、CMIA、PCR-荧光探针法和重组免疫印迹法(RIBA)检测,并对结果进行比较。结果丙型肝炎病例组的ELISA,CMIA及PCR-荧光探针法检测阳性率高于非丙型肝炎对照组,RIBA则较低。CMIA的灵敏度、特异度、阳性预测值、阴性预测值、符合率和约登指数分别为95.3%、95.5%、92.4%、97.3%、95.4%和90.8%。CMIA与ELISA比较,CMIA的特异度、阳性预测值、符合率和约登指数比ELISA高,差异有统计学意义(P<0.05)。CMIA与PCR比较,CMIA的灵敏度、阴性预测值、符合率和约登指数比PCR-荧光探针法高,差异有统计学意义(P<0.05),特异度比PCR-荧光探针法低,差异有统计学意义(P<0.05)。CMIA与RIBA比较,CMIA的灵敏度、阴性预测值和约登指数比RIBA高,差异有统计学意义(P<0.05),特异度比RIBA低,差异有统计学意义(P<0.05)。结论 CMIA具有高的灵敏度和阴性预测值,较高的特异度和阳性预测值,符合率高,约登指数高且诊断价值高。
Objective To research the application value of chemiluminescent microparticle immunoassay in the diagnosis of Hepatitis C. Methods From April 2014 to April 2015,we collected 350 cases of serum samples from the first affiliated hospital of Xinjiang medical university. According to the clinical diagnosis, the patients were divided into observation group （128 cases） and the control group （222 cases）. Eenzyme linked immunosorbent assay （ELISA）, chemiluminescence microparticle immunoanalysis （CMIA）,PCR fluorescence probe method and recombinant immunoblotting （RIBA） detection were used in this experiment. Results The positive of RIBA in control group was higher than that of observation group,while other measures turned to be the opposite. The sensitivity, specificity, PV＋, PV--, coincidence rat and Youden index of CMIA method were 95.3 %, 95.5 %, 92.4 %, 97.3 %, 95.4% and 90. 8% respectively. The Specificity, PV＋, coincidence rate and Youden index of CMIA were higher than those of ELISA,and the difference was statistically significant （P〈 0. 05）. The sensitivity, PV--, coincidence rate and Youden index of CMIA were higher than those of PCR,and the difference was statistically significant （P〈0.05）. The specificity of CMIA was lower than that of PCR, and the difference was statistically significant （P〈0.05）. Compared with RIBA, the sensitivity, PV- and Youden index were higher,and the difference was statistically significant （P〈0.05） ;the specificity were lower, and the difference was statistically significant （P〈0.05）. Conclusion Chemiluminescent microparticle immunoassay analysis has high sensitivity, negative predictive value, higher specificity and positive predictive value,high coincidence rate, Youden index,and diagnostic value.
出处
《重庆医学》
CAS
北大核心
2016年第23期3226-3228,共3页
Chongqing medicine
基金
卫生部医药卫生科技发展研究中心专项课题(28-1-13)
关键词
肝炎
丙型
分子探针
聚合酶链反应
酶联免疫吸附测定
化学发光微粒子免疫分析
PCR-荧光探针法
Hepatitis C I molecular probes
polymerase chain reaction
enzyme-linked immunosorbent assay
chemilumines- cent micr0particle immunoassay
PCR-fluorescence probing
