摘要
目的:研究大孔树脂纯化甘草地上部分水提液中总黄酮的工艺。方法:通过考察D-101、AB-8、DM-130、HPD-BJQH、HPD-600、HPD-100和HPD-450等7种树脂对总黄酮的静态吸附与解吸附,优选适宜类型的大孔树脂,并进一步对富集纯化参数进行考察及优化。结果:选用AB-8型大孔树脂,优选的工艺条件为:上样液质量浓度2.128 mg·m L-1,样品液p H5.42,树脂的上样量为7 m L/g,上样速度为1.5 BV,依次用2 BV水洗脱,4BV60%乙醇洗脱,经AB-8树脂处理后的甘草地上部分水提液的总黄酮纯度由9.08%提升到34.02%,收率为74.40%。结论:AB-8型大孔树脂能有效地纯化富集甘草地上部分水提液总黄酮,且此工艺稳定。
Objective：To investigate the purification technology of total flavonoids of water extract from the aerial parts of licorice. Methods：Seven macroporous resins（D-101,AB-8,DM-130,HPD-600,HPD-100 and HPD-450）were chosen with static and dynamic adsorption and desorption experiments to optimize the purification parameters. Results：AB-8 macroporous resin was chosen and its optimized technology conditions were as followings：the concentration of sample solution 2.128 mg·m L-1（p H=5.42）,the loaded amount of 7 m L/g,and loading flow rate of 1.5 BV/h. The sample was eluted by distilled water with 2 BV and 60% ethanol of 4 BV,respectively. The purity of total flavonoids was increased from 9.08% to 34.02% after the purification,and the yield was 74.40%. Conclusion：AB-8 is an ideal resin with the best enrichment for separating and purifying the total flavonoids of water extract from the aerial parts of licorice.
出处
《辽宁中医药大学学报》
CAS
2016年第8期60-63,共4页
Journal of Liaoning University of Traditional Chinese Medicine
基金
国家“重大新药创制”科技重大专项项目(2009ZX09308-002)
国家中医药管理局行业专项项目(201107009-03)
关键词
甘草
地上部分
水提液
总黄酮
大孔树脂
纯化工艺
licorice
aerial parts
water extract
total flavonoids
macroporous resin
purification technology
