摘要
目的:建立高效液相法测定复方丹七郁胶囊中丹酚酸B含量的方法。方法:采用Kromasil 100-5C18(250mm×4.6mm,5μm)色谱柱;以甲醇-乙腈-甲酸-水(10∶30∶1∶59)为流动相,波长为286nm;流速为1.0m L·min^(-1);柱温为30℃;进样量为10μL。结果:丹酚酸B在6.25~249.87μg·m L^(-1)范围内呈良好的线性关系,平均回收率100.51%(RSD为0.3%,n=6)。结论:该法快速,简便易行,结果可靠,专属性强,可作为复方丹七郁胶囊的质量控制指标。
Objective：To develop a high performance liquid chromatography（HPLC）method for simultaneous determination of salvianolic acid B in Compound Dan Qi Yu jiangzhi Capsule. Methods：Samples were analyzed on Kromasil 100-5C18（250mm ×4.6mm,5μm）.Salvianolic acid B was detected with a mobile phase of methanol- acetonitrile-methanoic acid-water（10：30：1：59）for isocratic elution and a detection wavelength of 286 nm. The flow rate was 1.0m L·min-1,temperature of column was set at 30℃ and injection volume was10μL. Results：Salvianolic acid B showed good linearity in the range of 6.25 ~249.87μg·m L-1,and the recovery was 100.51%（RSD =0.3%,n =6）.Conclusion ：The established method is rapid,practical and accurate,and is suitable for the quanlity control of Fufang Dan Qi Yu Capsule.
出处
《北方药学》
2016年第9期2-3,共2页
Journal of North Pharmacy
基金
复方丹七郁胶囊工艺制备和质量标准研究
项目编号:20161245
