摘要
背景:传统修复胸壁缺损的材料由于无法降解,需要长期留在体内且组织反应较大,并发症发生率较高,难以满足修复的需要。目的:制备生物可降解网状人工胸壁,分析其性能及在胸壁缺损中的应用效果。方法:将蚕丝纤维来源与聚己内酯熔融共混,制备生物可降解网状人工胸壁材料,检测其孔隙率与弹性模量。取22只家兔,建立胸壁缺损模型,随机分2组,实验组置入生物可降解网状人工胸壁材料,对照组置入聚己内酯材料,置入后4周,苏木精-伊红染色观察缺损部位;置入后2个月,CT检查骨缺损部位。结果与结论:(1)生物可降解网状人工胸壁材料孔洞分布较均匀,孔隙率为54.4%,直径200-300μm,材料中纤维分布相对稳定,弹性模量高于聚己内酯(P<0.05);(2)CT显示,实验组材料与肋骨断端紧密接触,胸壁板与脏层胸膜之间出现新生组织;对照组材料与断端处接触,边界不清,存在高密度影;(3)苏木精-伊红染色显示,实验组有大量纤维细胞,胶原纤维较疏松,炎细胞数少;对照组纤维肌膜与肌肉边界不清,存在水肿,有较多炎细胞浸润;(4)结果表明:生物可降解网状人工胸壁可促进缺损胸壁的修复。
BACKGROUND：Due to their inabilityto be degraded, the traditional repair materials for chest wal defects require a long-term stay in the body. Therefore,severetissue reaction and the high incidence of complications make the traditional materialsunable tomeet the requirements of restoration.
OBJECTIVE：To prepare an artificial reticular chest wal using biodegradable materials, and to analyze its performance and application in therepair ofchest wal defect.
METHODS：Mixture ofsilk fiber and polycaprolactonewas usedto prepare thebiodegradablechest wal, and its porosity and modulus of elasticity were measured. Twenty-two rabbits were selected to build chest wal defect models, which were randomized into two groups. The artificial reticular chest wal was implanted into experimental group, and polycaprolactone implanted into control group. At 4 weeks after implantation, the chest defect was observed by hematoxylin-eosin staining; at 2 months, the chest defect regionwas observed by CT examination.
RESULTS AND CONCLUSION：The holeswereuniformlydistributedin the artificial chest wal,withthe porosityof54.4%andthe diameterof200-300 μm, fiber distribution in the material was relatively stable, and the modulus of elasticity was significantly higher than that of the polycaprolactone （P〈 0.05）. CT showed that in the experimental group,theimplantmaterial was in close contact with the rib ends, and new tissues appeared between the chest wal plate and visceral pleura;in the control group,the material contacted thebrokenendswith unclear border, anda visible high density. Hematoxylin-eosin staining showed that in the experimental group there were numerous fibroblasts and a smal amount of inflammatory cels, and colagen fibers were loose;in the control group,the boundary between the muscle fibermembrane and the musclebecameobscure,accompanied by appearance ofedema and more inflammatory cels. These resultsshowthat the artificial reticular chest walmade ofbiodegradable materials can promote the chest wal repair.
出处
《中国组织工程研究》
CAS
北大核心
2016年第34期5064-5069,共6页
Chinese Journal of Tissue Engineering Research
