摘要
目的探讨聚合酶链式反应（PCR）反向点杂交法检测人乳头瘤病毒（HPV）分型在确定早期宫颈癌中的价值。方法选取2013年1月-2014年12月2 228例自愿参与宫颈筛查的已婚或有性生活史的女性为研究对象,采用PCR反向点杂交法检测患者宫颈分泌物中HPV感染情况及分型,并对筛查者行液基薄层细胞检测（TCT）,以病理组织学诊断为金标准,对比分析PCR与TCT诊断在宫颈病变早期筛查中的应用价值。结果 2 228例受试者中HPV检出率为21.77%（485/2 228）,各种类型的HPV感染率前5位依次为HPV16、HPV31、HPV58、HPV18、HPV52,其中重叠感染率为5.48%（122/2 228）。组织病理学宫颈病变阳性率为11.04%（246/2 228）,TCT宫颈病变阳性率为15.53%（346/2 228）。TCT阳性患者中HPV阳性率为61.56%（213/346）,病理组织学检测阳性中HPV阳性率为81.30%（200/246）。经分析可知,与PCR或TCT相比,PCR＋TCT诊断灵敏性及特异性较高,差异有统计学意义（χ2值分别为32.495、79.36,P〈0.05）。结论对行TCT的患者同时行PCR反向点杂交法检查,将有助于提高TCT诊断灵敏性及特异性,对早期宫颈鳞状细胞癌诊断具有重要的价值。
Objective To explore the value of human papillomavirus（ HPV） typing by PCR reverse dot blot hybridization in determining early stage cervical cancer. Methods A total of 2 228 married women or women having sexual life history underwent cervical screening voluntarily from January 2013 to December 2014,PCR reverse dot blot hybridization was used to detect HPV infection status and typing in cervical secretions,liquid- based cytology test（ TCT） was conducted,taking pathohistological examination result as gold standard,the application values of PCR and TCT in early screening of cervical lesions were compared and analyzed. Results The detection rate of HPV was21. 77%（ 485 /2 228）,the top five HPV infection subgroups were HPV16,HPV31,HPV58,HPV18,and HPV52,respectively,the mixed infection rate was 5. 48%（ 122 /2 228）. The positive rate of cervical lesions detected by pathohistological examination was 11. 04%（ 246 /2 228）. The detection rate of cervical lesions by TCT was 15. 53%（ 346 /2 228）,among the patients with positive result of TCT,the positive rate of HPV was 61. 56%（ 213 /346）; among the patients with positive result of pathohistological examination,the positive rate of HPV was 81. 30%（ 200 /246）. Compared with single application of PCR or TCT,the sensitivity and specificity of joint detection of PCR and TCT were higher,there were statistically significant differences（ χ2= 32. 495,79. 36,P〈0. 05）. Conclusion PCR combined with TCT can improve sensitivity and specificity of TCT diagnosis,which has important value for diagnosis of early cervical squamous cell carcinoma.
出处
《中国妇幼保健》
CAS
2016年第16期3244-3246,共3页
Maternal and Child Health Care of China
关键词
PCR反向点杂交法
人乳头瘤病毒
早期宫颈癌
临床价值
PCR reverse dot blot hybridization
Human papillomavirus
Early cervical cancer
Clinical value
