摘要
[目的]ENH1是最近鉴定的拟南芥耐盐基因,编码一个叶绿体定位的蛋白,N末端具有一个PZD结构域,C末端具有一个RUBR结构域;功能上与活性氧自由基的脱毒有关,本文研究ENH1基因的进化历史和功能分化。[方法]从陆地植物基因组中获得同源蛋白,重建这个基因家族的系统发生,通过生物信息学手段研究其蛋白质结构域组织,蛋白质相互作用和表达谱。[结果]总共获得35个ENH1同源蛋白序列,大多数物种保持一个基因拷贝,系统发育上单子叶与双子叶被明确划分,并获得高度的支持,在进化过程EHN1蛋白获得了PDZ结构域。[结论]功能上,这个基因家族通过获得PZD结构域适应被子植物细胞的复杂功能要求,ENH1样基因可能涉及叶绿体的多种功能,此外,ENH1基因家族适合作为系统发育重建的分子标记。
[ Objective] To investigate the evolutionary history and functional differentiation of the ENH1 gene family that was recently characterized as a salt tolerance gene that encodes a chloroplast - localized protein with a PDZ domain at the N - termi- nal region and a rubredoxin （RUBR） domain in the C - terminal part ,functionally responsible for detoxification of reactive oxy- gen species resulting from salt stress. [ Methods ] ENH1 homologous proteins were obtained from land plants genomes. Bioinfor- matics approaches were used for investigating phylogeny, protein domain organizations, and protein network and expression pro- file. [ Results ] A total of 35 ENH1 homologous proteins were obtained. It was found that most of plant species maintain one gene copy, and all ENH1 -like genes were phylogenetically divided into dicots and monocots clades with strong bootstrap supports. EHN1 -like proteins obtained the PDZ domain during evolution. [ Conclusion] ENH1 genes adapted for the complex require- ment in angiosperm cells through obtaining the PDZ domain. ENH1 genes might participate in multiple chloroplast function. In addition, ENH1 genes were well suitable for phylogenetic reconstructing.
出处
《生物技术》
CAS
CSCD
北大核心
2016年第4期348-352,324,共6页
Biotechnology
基金
江苏省自然科学基金面上项目("利用系统发育基因组学方法鉴别生物固氮基因的研究"
No.BK20151269)
江苏省大学生实践创新训练计划项目("耐盐基因NKS1的系统发生与协同进化研究"
No.201510304058Y
"根瘤固氮基因网络的进化"
No.201610304087Y)
