摘要
目的探讨miR-299-3P在早期梅毒及血清固定患者外周血单核细胞(PBMC)中的表达及临床意义。方法收集2010年1月-2016年1月期间在该院皮肤性病中心诊断的早期梅毒、血清固定患者各35例,并收集35例健康志愿者作为对照组,QRT-PCR检测患者及健康志愿者PBMC中miR-299-3P水平;同时对血清固定患者miR-299-3P水平与RPR滴度及临床分期进行相关分析。结果梅毒血清固定患者外周血miR-299-3P水平明显高于早期梅毒及健康志愿者,差异有统计学意义(P<0.05)。血清固定患者血清miR-299-3P水平与血清初始RPR固定滴度相关(P<0.05),与临床分期无关(P>0.05)。结论外周血单核细胞中miR-299-3P表达水平增高可能与梅毒血清固定相关。
Objective To investigate the expression and clinical significance of miR-299-3P in peripheral blood mononuclear ceils （PBMC） in early and serofast syphilis patients. Methods Blood samples were collected from 35 healthy controls and 35 patients with early syphilis and serofast syphilis at dermatology of our hospital from January 2010 to January 2016 . QRT-PCR was used to measure the expression levels of miR-299-3P in PBMC. The relationship of miR-299-3P with RPR titer and clinical stage were also analyzed. Results The levels of miR-299-3P in PBMC of patients with serofast syphilis were significantly higher than that with early syphilis and healthy volunteers （ P 〈 0. 05 ）. The levels of miR-299-3P was correlated with the initial RPR fixed titer （ P 〈 0. 05 ） , but not clinical stage （ P 〉 0. 05 ）. Conclusion The higher expression of miR-299- 3P in serum may be related to the early stage of syphilis.
出处
《中国皮肤性病学杂志》
CAS
CSCD
北大核心
2016年第9期924-926,共3页
The Chinese Journal of Dermatovenereology
