摘要
目的:纹状体神经元可以投射突触到室管膜下区(SVZ),并影响SVZ区域前体细胞的活性,但是纹状体神经元活性如何影响SVZ区域神经再生未知,本文旨在探讨纹状体神经元与SVZ区域神经再生的关系。方法:利用光遗传学的方法,特异调控纹状体神经元活性后,利用免疫组织化学方法观察其活性对SVZ区域神经再生的影响,并且探讨其可能机制。结果:当以CaM KⅡ作为启动子时,光敏感蛋白可以在纹状体神经元中表达,而且纹状体神经元的发放频率能够被激光控制。纹状体神经元活性的抑制促进NT-1的分泌(P<0.05),免疫组织化学结果显示纹状体神经元活性的降低促进SVZ区域BrdU与DCX双阳性细胞数目的增多(P<0.05)。结论:纹状体神经元活性是影响SVZ区域神经再生的重要因素,抑制纹状体神经元活性可以促进神经再生。
Objective： Neurons in the striatum project axons to the SVZ,and can influence the activity of the progenitor cells in the SVZ. However,the relation between striatal neurons and SVZ neurogenesis are still unknown. The aim of the current study was to investigate the relation between striatal neurons and SVZ neurogenesis. Methods： optogenetics was used to specifically regulate the activity of the striatal neurons and investigated the neurogenesis progress in SVZ as well as the mechanisms. Results： The results showed that most of the striatal neurons can be transfect by injection of lentivirus carried by promoter CaM KⅡ. Lasers could regulate the spikes of striatal neurons. In addition,inhibition the activity of striatum neurons increased the expression of NT-1（ P 0. 05） and the BrdU / DCX double positive cells（ P 0. 05）. Conclusion： We conclud that the activity of striatal neurons is an important factor in regulating neurogenesis in SVZ,and inhibition of striatal neurons promote neurogenesis.
出处
《神经解剖学杂志》
CAS
CSCD
北大核心
2016年第4期466-470,共5页
Chinese Journal of Neuroanatomy
基金
国家自然科学基金(81501014)
