摘要
目的:探讨杨梅素对佐剂性关节炎(adjuvant arthritis,AA)小鼠的原发性关节滑膜病理以及血液炎症因子的影响。方法:将弗氏佐剂造模成功的小鼠,随机分为正常对照组、模型组、阳性对照组、杨梅素高剂量组5 mg/kg、低剂量组1 mg/kg。于致炎12 d后,杨梅素组每天灌胃给予相应剂量杨梅素,阳性对照组给予地塞米松,正常组、模型组给予等体积生理盐水。每隔4 d测量小鼠的体重、足体积并评价关节炎指数,于第30 d处死小鼠后取胸腺、脾称重计算脏器指数,取关节苏木-伊红(HE)染色法观察滑膜病理,酶联免疫(ELISA)法测量血清炎症因子水平。结果:与正常组相比,模型组小鼠的关节肿胀率显著增加,胸腺、脾指数增加,血清炎症因子显著增加,且具有统计学意义(p<0.05或p<0.01),滑膜增生严重并伴有大量炎症细胞浸润;与模型组相比杨梅素高、低组小鼠关节肿胀率显著下降,胸腺指数下降,血清炎症因子质量浓度降低,具有统计学意义(p<0.05或p<0.01),脾指数下降不明显,滑膜无明显增生,炎症浸润程度也减轻。结论:杨梅素具有改善类风湿性关节炎发病过程中炎症反应的作用,其机制可能与抑制免疫器官功能亢进以及炎症因子的质量浓度,改善滑膜病变有关。
Objective：To explore the effects of myricetin on pathology of synovialis and inflammatory factors of mice with adjuvant arthritis. Methods： Mice induced by Freunds complete adjuvant were randomly divided into five groups：normal,model, high dose myricetin, low dose myricetin, positive control （Dexamethasone）.The myricetin group was given myricitin by ig 12 days after inducing.The weight and foot volume was measured every 4 days, arthritis index was evaluated after measuring foot volume.Execute mice at the 30th day,thymus and spleen were weighted to count organ index, took the knee joint for pathological examination using hematoxylin- eosin staining.inflammatory factors was measured using ELISA kits.Results：The degree of swelling,thymus and spleen index, inflammatory factors were all increased in the model group compared with the normal group（ p 〈 0.05 or p 〈 0.01）.The degree of swelling, thymus index, inflammatory factors were all decreased in the myricetin groups compared with the model group （p 〈 0.05 or p 〈 0.01 ）, inflammatory ceils infiltration of synovialis was sigllificantly slighter than model group.Conclusion： Myricetin can inhibit the immune organs and decreased the levels of inflammatory factors,that may be related to myricetin＇ s effect on inhibiting rheumatoid arthritis.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第17期340-343,共4页
Science and Technology of Food Industry
基金
国家自然科学基金(81273127)
关键词
杨梅素
类风湿性关节炎
滑膜病理
炎症因子
Myricetin
rheumatoid arthritis
pathology of synovialis
inflammatory factors
