摘要
目的:研究柑橘多糖对小鼠佐剂性关节炎模型(AA)的滑膜病理和炎症因子表达的影响。方法:随机将雌性昆明小鼠分为正常对照组,模型组,柑橘多糖高、低剂量组,地塞米松组。各组造模后12 d灌胃给药,1次/d,连续14 d。正常对照组,模型组灌胃等体积生理盐水。分别采用足容积法和5级评分法测量小鼠原发侧足肿胀度及关节炎指数,并计算足肿胀率,测定胸腺、肝指数。HE染色法检测小鼠滑膜病理。ELISA法测定血清中炎症因子含量。结果:与正常组比较,模型组小鼠的体重显著下降,关节肿胀率,胸腺、脾指数以及血清中炎症因子含量显著增加,具有统计学意义(p<0.01或p<0.05),且模型组的关节滑膜组织增厚,伴大量炎症细胞浸润;与模型组比较,高剂量组能显著提高小鼠体重,降低小鼠关节肿胀率,胸腺、脾指数以及血清炎症因子含量,具有统计学意义(p<0.01或p<0.05),且高剂量组能显著改善滑膜病理,减少炎症细胞的浸润。低剂量组有一定效果,但效果不明显,相对于高剂量组无治疗意义。结论:适宜浓度的柑橘多糖对AA有抑制作用,其机制可能与柑橘多糖通过下调血液炎症因子,减轻滑膜组织炎性细胞浸润,发挥抗炎作用和通过降低AA小鼠胸腺指数,发挥免疫调节作用有关。
Objective：To investigate the influence of citrus polysaccharide on the synovium pathomorphology and the production of inflammatory cytokines in mice with AA.Methods, Female mice were randomly divided into normal control group,model group, citrus polysaccharide high and low dose groups, dexamethasone group.Each group were stated to give medicine 12 days after model establishing, medicine were given once daily for continue 14 days. The normal and model groups were given a gavage of normal saline water of the same volume with the drug.Foot volume method was adopted to measure paw swelling of mice and five grading evaluations was used to evaluate arthritis index of mice, then the swelling rate, thymus index and liver index were calculated.Synovium pathomorphology was measured by HE,and inflammatory factor was measured by ELISA.Results.Compared with the normal group, the model mice＇ s weight were decreased, the swelling rate, thymus, spleen index and inflammatory factor were all increased. （p 〈 0.01 or p 〈 0.05）, and the synovial proliferation and hyperplasia enlargement of the tissue were all raised.Compared with the model group, the citrus polysaccharide high dose mice＇ s weight were increased,the swelling rate,thymus, spleen index and inflammatory factor were all decreased. （p 〈 0.0l or p 〈 0.05）, and the synovial proliferation and hyperplasia enlargement of the tissue were all reduced. Low-dose group had some effect, but the effect is not obvious, with respect to the high-dose group had no therapeutic significance Conclusions. citrus polysaccharide of suitable concentrations could suppress the AA mice.Its active mechanisms may be related to decrease the levels of inflammatory factors and infiltration of inflammatory cells of AA mice,and active immunomodulatory by reduce thyrmus weight.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第17期352-355,371,共5页
Science and Technology of Food Industry
关键词
柑橘多糖
佐剂性关节炎
滑膜病理
炎症因子
citrus polysaccharide
Adjuvant Arthritis
synovium pathomorphology
inflammatory factors
