摘要
目的探讨中药促愈熏洗方对大鼠背部早期创面愈合的影响,并通过实验研究其促进愈合的机制。方法选取Wistar大鼠制成全层皮肤缺损模型,随机分为中药组(促愈熏洗方)6只、西药组(利凡诺)6只、空白对照组(0.9%Na Cl溶液)6只,进行相应干预。分别观察各组创面愈合情况,并用Western blotting法检测创面组织病理改变VEGF、VEGFR-1、VEGFR-2及血清酶联免疫法(ELISA)检测TNF-α、IL-6的表达变化。结果中药组创面愈合时间与西药组基本相同,均短于空白对照组,造模后第3 d各观察指标无统计学意义(P>0.05);第7、15 d促愈熏洗方组创面愈合率及肉芽生长情况、血清TNF-α、IL-6水平均优于空白对照组(P<0.05)。第7 d,中药组创面愈合率高于西药组(P<0.05)。各组VEGF、VEGFR-1、VEGFR-2表达差异均无统计学意义(P>0.05)。结论促愈熏洗方可明显抑制创面早期炎症因子TFN-α和IL-6的表达,从而促进创面的愈合。
Objective To investigate the effect of Chinese Herbal Fumigant of ＂ Cuyu Xunxi Formula＂ on wound healing in the early stage of rats and the mechanism of promoting healing based on experiment. Methods The whole layer skin defection model was established in Wistar rats. The rats were randomly divided into the following groups： Cuyu Xunxi Formula group, control group （rivanol）, and blank control group （0.9% saline）. The condition of wounds was observed. VEGF, VEGFR-1 VEGFR-2 expression in wound tissue was detected by Western blot, and expression of serum TNF-α and IL-6 was detected by ELISA. Results The healing time had no significant difference between Cuyu Xunxi formula group and control group, and was shorter than the blank control group. There was no statistical significance of the observation indexes on the third day after operation （ P 〉0.05 ）. The healing rate, granulation growth and serum TNF-α, IL-6 lever in Cuyu Xunxi Formula group were better than those of the blank control group on the seventh day and fifteenth day （ P 〈0.05 ）. On the seventh day, the wound healing rate in Cuyu Xunxi Formula group was higher than that in control group, and there was no significant difference in VEGF, VEGFR- 1 AND VEGFR-2 among all groups. Conclusion The pathogenesis of early wound based on exuberance of heat-toxin and blood-stasis obstruction, Cayu Xunxi Formula could obviously inhibit the expression of inflammatory factor TNF-α and IL-6 of early wound and promote wound healing.
出处
《上海中医药杂志》
2016年第7期76-79,共4页
Shanghai Journal of Traditional Chinese Medicine
基金
上海市卫计委中医药科研基金项目(2014LP099A)
上海市科委科研基金项目(13401902503)
上海市卫生局中医药事业发展三年行动计划项目(ZY3-JSFC-2-1037)
