摘要
目的评价纯镁和镁铜合金对粪肠球菌的体外抗菌效果。方法选取因正畸新鲜拔除的人的单根管下颌前磨牙40颗,随机分为4组,每组10颗牙。所有牙齿清理根管后灭菌,建立粪肠球菌感染根管模型。每组分别用以无菌生理盐水为赋形剂调制的氢氧化钙糊剂(A组)、镁粉糊剂(B组)、镁铜合金粉糊剂(C组)、生理盐水(D组)进行根管封药7天。封药前后分别取样,接种在普通营养琼脂平板上培养,计数菌落。结果封药前4组根管内细菌量的差异无统计学意义(P>0.05)。D组封药前后根管内细菌量无统计学差异(P>0.05)。A、B、C 3组封药后根管内细菌量均明显减少,与封药前细菌量比较,差异具有统计学意义(P<0.05)。A、B、C 3组间两两比较,封药后根管内细菌减少量差异无统计学意义(P>0.05)。结论纯镁和镁铜合金对粪肠球菌均有一定的抗菌效果。但各组根管内仍有粪肠球菌残留,关于镁和镁铜合金在口腔临床医学中的应用价值还需要进一步研究。
Objective To evaluate the antibacterial efficacy of pure magnesium （Mg） and Mg-Cu alloys using anin vitro model infected by Enterococcus Faecalis（Efaecalis）. Methods Forty freshly extracted human sin- gle-rooted mandibular premolars were collected and divided randomly into 4 groups, with 10 teeth in each group. After cleaning and sterilization, the root canals were infected with E. faecalis to establish the E. faecalis infected models. All root canals were dressed for 7 days with different intracanal medicament as follows： group A with calcium hydroxide paste plus sterile normalsaline; group B with magnesium （Mg） paste plus sterile normalsaline; group C with Mg-Cu alloys paste plus sterile normal saline; group D with sterile normal saline. Microbiological samples were collected from root canal before and after dressing, and were incubated at 37℃ and plated onto common nutrient agar .The colony of bacteria was counted （CFU/ml）. Results There was no statistically significant difference in the number of E. faecalis of all groups before root canal dressings （P〉0.05）. After dressing, no sig- nificant difference was observed in the number of E.faecalis in the group D （P〉0.05）. The number ofE. faecalis in group of calcium hydroxide, Mg and Mg-Cu alloys were effectively reduced （P〈0.05）. While there was no significant difference between every two groups of Group A,B and C （P〉0.05）. Conclusion The results suggested that Mg and Mg-Cu alloys paste plus sterile physiological saline were all effective for eliminating E. faecalis in root canal specimens. Further studies are needed to confirm the value of Mg and Mg-Cu alloys in clinical treatment.
出处
《口腔材料器械杂志》
2016年第3期139-143,共5页
Chinese Journal of Dental Materials and Devices
基金
辽宁省博士启动基金(编号:201501027)
关键词
纯镁
镁铜合金
氢氧化钙
抑菌
粪肠球菌
Magnesium Mg-Cu alloys Calcium hydroxide Bacteriosasis Enterococcus faecalis
