摘要
烟曲霉(Aspergillus fumigatus)是一种广泛存在于自然界中的条件致病菌,其产生的分生孢子被易感人群吸入后定植于肺部,引起3种曲霉病:致咯血的曲霉肿、致肺纤维化的变应性支气管肺曲霉病、致较高死亡率的侵袭性曲霉病。目前临床上用于诊断烟曲霉感染的血清免疫学指标有半乳甘露聚糖和1,3-β-D-葡聚糖,但特异度和灵敏度方面存在一定的局限性。Asp f3和Asp f4为烟曲霉主要抗原,在感染者血清中存在相应的循环抗体。本研究分别用兔抗Asp f3和Asp f4抗血清对其进行抗原表位扫描,鉴定了8个Asp f3和6个Asp f4最小表位基序肽。用所鉴定的最小表位基序与烟曲霉其他抗原的最小表位基序构建嵌合肽,可能有助于提高烟曲霉感染诊断的特异度和灵敏度。
Aspergillus fumigatus （ A. fumigatus ） is produced by A. furnigatus are colonized in lungs a widespread opportunistic pathogen. The conidia after inhalation, leading to aspergilloma, allergic bronchopulmonary aspergillosis （ABPA） and invasive aspergillosis （IA）. Currently, 1,3-β-D-glucan and galactomannan are used as serum immunological indexes for clinical diagnosis of A. fumigatus infection. Asp f3 and Asp f4 are two of the major antigens of A. fumigatus, and the corresponding circulating antibodies are present in the sera of infected persons. In this study, anti-Asp f3 and anti-Asp f4 rabbit sera were used to carry out antigen epitope scanning. Eight ASp f3 and six ASp f4 minimal motifs were identified. These identified minimal motifs can be used for constructing chimera antigens to improve the specificity and sensitivity of the diagnosis for A. fumigatus infection.
出处
《微生物与感染》
2016年第4期240-246,共7页
Journal of Microbes and Infections
基金
国家重点基础研究发展计划(2013CB531603)
上海市科学技术委员会项目(13DZ2252000)
