摘要
本文研究了批次培养模式下培养基中分别添加0.018 mol/L不同碳源(Na HCO_3,C6H12O6,CH_3COONa和CH_3CH_2COONa)对球等鞭金藻(Isochrysis galbana)生长、色素含量、蛋白质含量和脂肪酸组成的影响。结果表明:在盐度26,温度25℃,光照3000 lux条件下,f/2培养液中添加不同碳源对球等鞭金藻的生长和细胞组成有显著影响。不同碳源对球等鞭金藻促生长效果依次为:葡萄糖>碳酸氢钠>空白对照>丙酸钠>乙酸钠。培养液中添加各种碳源均不同程度上降低了球等鞭金藻细胞蛋白质含量。添加碳酸氢钠、乙酸钠和丙酸钠组藻叶绿素a和类胡萝卜素含量均显著降低,而添加葡萄糖组藻细胞中两种色素含量显著增加。添加碳源改变了球等鞭金藻细胞脂肪酸组成,单不饱和脂肪酸(MUFA)含量均显著增加,而多不饱和脂肪酸(PUFA)均显著降低。上述研究表明葡萄糖是促进球等鞭金藻生长的高效碳源,培养液中添加葡萄糖是球等鞭金藻高密度培养的有效策略。
This paper studied the effect of adding 0.018 mol/L of NaHCO3, C6H12O6, CH3COONa and CH3CH2COONa intomedium respectively on the growth, cellular pigment and protein contents and fatty acid profiles of the microalgae Isochrysisgalbana under a batch culture model. The results showed there was a significant influence to add different carbon compoundsinto f/2 medium under temperature 25 ℃, light intensity 3000 lux, salinity 26. The growth promoting effect of the carbonsources were as follows： glucose 〉NaHCO3〉CH3CH2COONa〉CH3COONa. The cellular protein contents in microalgaedecreased when the carbon sources were supplemented in the medium. The treatments supplementedNaHCO3,CH3CH2COONa and CH3COONa displayed significant low Chlorophyll a and Carotenoids contents. The treatmentssupplemented glucose displayed significant low chlorophyll a and carotenoids contents. The fatty acid profiles of themicroalgae also changed when different carbon sources were added into the medium. The monounsaturated fatty acids contentincreased while polyunsaturated fatty acids content decreased significantly. It was therefore suggested that glucose was aneffective carbon source for improving the growth of the microalgae and could be applied in the production of microalgae.
出处
《山东农业大学学报（自然科学版）》
CSCD
2016年第4期506-513,共8页
Journal of Shandong Agricultural University：Natural Science Edition
基金
上海市科技兴农项目(沪农科推字(2013)第2-1号)
上海市科技兴农项目(沪农科攻字(2015)第1-2号)
上海高校水产学一流学科建设项目资助
关键词
球等鞭金藻
碳源
葡萄糖
细胞组成
Isochrysis galbana
carbon source
glucose
cellular profiles
