摘要
为探讨辣椒碱对金黄色葡萄球菌感染小鼠淋巴细胞和巨噬细胞免疫调节活性的影响,采用MTT法检测辣椒碱对金黄色葡萄球菌感染的淋巴细胞增殖的影响;ELISA法检测辣椒碱对金黄色葡萄球菌感染的淋巴细胞诱导IFN-γ、IL-6分泌的影响,以及辣椒碱对金黄色葡萄球菌感染的巨噬细胞诱导TNF-α、IL-6分泌的影响。结果表明,辣椒碱在一定浓度范围内,能促进对金黄色葡萄球菌感染的淋巴细胞的增殖和诱导IFN-γ、IL-6的分泌,且呈双向调节;能诱导金黄色葡萄球菌感染的巨噬细胞TNF-α、IL-6的分泌,从而发挥免疫调节作用。
The effect of capsaicin on immunoregulatory activity of mice lymphocytes and macrophages by Staphylococcus aureus infection was studied.The effect of capsaicin on lymphocyte proliferation by Staphylococcus aureus infection was studied using MTT;and the effect of capsaicin on lymphocytes inducing IFN-γand IL-6secretion by Staphylococcus aureus infection was studied using ELISA,as well as the effect of capsaicin on macrophages induced TNF-αand IL-6secretion.Results showed that：capsaicin within a certain range of concentrations,can promote lymphocyte proliferation and induction of IFN-γand IL-6secretion by Staphylococcus aureus infection,and the biphasic regulation;and induce macrophages secretion of TNF-αand IL-6by Staphylococcus aureus infection,which played a role in immune regulation.
出处
《粮食与饲料工业》
CAS
2016年第8期44-48,56,共6页
Cereal & Feed Industry
基金
辽宁省科技攻关计划"辽宁省重大动物疫病防控与畜禽产品安全监控检测技术研究"(2014214016)
关键词
辣椒碱
淋巴细胞
巨噬细胞
增殖
分泌
免疫调节
capsaicin
lymphocytes
macrophages
proliferation
secretion
immunoregulatory
