摘要
目的探讨芍药苷对脑外伤大鼠模型行为学及下丘脑-垂体-肾上腺(HPA)轴的影响。方法将45只大鼠随机分为假手术组(n=15)、脑外伤组(n=15)、芍药苷组(n=15)。按照改良Feeney法制备脑外伤大鼠模型。芍药苷组术后3 h给予芍药苷2.0 mg/kg腹腔注射,假手术组不进行任何处理,脑外伤组注射等量生理盐水,2次/d。24 h后处死。采用定位航行试验和空间探索试验评价行为学,采用酶联免疫法测定下丘脑CRH和血清CS含量。结果与假手术组比较,脑外伤组、芍药苷组第1周和第2周大鼠逃避潜伏期明显延长(P<0.05),而芍药苷组第4周大鼠逃避潜伏期比较无统计学差异(P>0.05);与脑外伤组比较,芍药苷第1、第2、第4周大鼠逃避潜伏期明显降低(P<0.05);与假手术组比较,脑外伤组、芍药苷组第1、第2、第4周大鼠游泳时间百分比明显减少(P<0.05);与脑外伤组比较,芍药苷组第1、第2、第4周大鼠游泳时间百分比明显增加(P<0.05);与假手术组比较,脑外伤组、芍药苷组下丘脑CRH及血清CS含量明显上升(P<0.05);与脑外伤组比较,芍药苷组下丘脑CRH及血清CS含量明显降低(P<0.05)。结论芍药苷可明显改善脑外伤大鼠模型行为学,且对脑外伤大鼠模型HPA轴具有一定的调节作用。
Objective To investigate the effect of the peony of the rat model of brain injury induced by cerebral trauma and the hypothalamic pituitary adrenal（ HPA） axis.Methods Forty- five rats were randomly divided into 3 groups,sham operation group（ n = 15）,brain injury group（ n = 15）,and peony group（ n = 15）.A rat model of traumatic brain injury was prepared by modified Feeney method.Group of peony postoperative 3h treatment with intraperitoneal injection of 20 mg / kg,sham operation group was not treated with any treatment;the cerebral trauma group was injected with equal amount of saline,2 times a day.Execution was conducted after 24 h.Behaviors of the test were evaluated with place navigation test and pace exploration experiments,the contents of CRH in hypothalamus and CS in serum were determined by ELISA.Results Compared with the sham operation group,brain injury group and paeoniflorin group 1 week and 2 weeks in rats escape latency was significantly longer（ P〈0.05）,while paeoniflorin group and sham operation group at 4 weeks was no statistic difference（ P〉0.05）;compared with brain injury group,paeoniflorin group 1 week,2 weeks,4 weeks in rats escape latency was significantly lower（ P〈0.05）;compared with the sham operation group,brain injury group and paeoniflorin group 1week,2 weeks,4 weeks swimming time significantly reduced the percentage of rats（ P〈 0.05）;compared with brain injury group,paeoniflorin group 1 week,2 weeks,4weeks rat swimming time the percentage increased significantly（ P〈0.05）;compared with the sham operation group,brain injury group and paeoniflorin group hypothalamic CRH and serum CS were significantly increased（ P〈0.05）;compared with brain injury group,paeoniflorin group hypothalamic CRH and CS serum decreased significantly（ P〈 0.05）.Conclusion Peony can improve the behavior of rats with brain injury,and has a certain regulation effect on the HPA axis of brain injury model rats.
出处
《中国地方病防治》
CAS
北大核心
2016年第5期496-497,共2页
Chinese Journal of Control of Endemic Diseases
基金
海南省自然科学基金项目编号:琼科813210
