摘要
目的制备一种新型的硫醇修饰的磁性银花纳米粒子SERS基底对氯霉素(CAP)进行拉曼检测,确定SERS基底的增强效果。方法选择烷基硫醇作为基底的表面修饰剂,自组装到磁性银花纳米粒子的银壳表面,通过疏水作用,将CAP分子富集到基底表面,从而实现检测CAP分子拉曼信号的增强。结果 3种不同硫醇修饰的基底对CAP的增强顺序是正己硫醇>十二硫醇>十八硫醇。利用Fe_3O_4@SiO_2-Ag-C6 SERS基底对浓度范围10^(-3)~10^(-10)mol/L的CAP溶液和10^(-3)~10^(-9)mol/L的牛奶中CAP进行拉曼检测,检测限分别为0.1 nmol/L(32 ppt)和1 nmol/L(323 ppt)。结论硫醇修饰的磁性银花纳米粒子是一种高活性的SERS基底,可用于低浓度物质的检测分析。
Objective To prepare novel alkanethiol modified magnetic silver flower nanoparticles as SERS substrate to chloramphenicol for Raman detection and to determine their enhancement effect. Methods An alkanethiol was chosen as a surface modifier of the substrate and was self-assembled onto the magnetic silver flower nanoparticle surface. The chloramphenicol molecules were enriched to the surface of the substrate by hydrophobic interaction and the effect for detection of chloramphenicol SERS signal was enhanced. Results It was found that the 1-hexanethiol-modified SERS substrate was able to lead to stronger enhancement than 1-dodecanethiol and octadecanethiol. Fe3O4@SiO2-Ag-C6 was used to detect the chloramphenicol（ 10 -3- 10 -10 mol / L） and chloramphenicol in milk（ 10 -3- 10 -9mol / L） by surface-enhanced Raman spectroscopy. The detection limits were 0. 1 nmol / L（ 32 ppt） and 1 nmol / L（ 323 ppt） respectively. Conclusion Alkanethiol modified magnetic silver flower nanoparticles are a highly active SERS substrate,which can be used for detection of low concentrations of analytical substances.
出处
《军事医学》
CAS
CSCD
北大核心
2016年第8期634-638,共5页
Military Medical Sciences
基金
国家重大科学仪器设备开发专项资助项目(2012YQI80117)
关键词
光谱分析
拉曼
硫醇
磁性
纳米粒子
氯霉素
spectrum analysis
Raman
alkanethiol
magnetic
nanoparticles
chloramphenicol
