摘要
香蒲(Typhaangustifolia L.)是一种重金属镉富集植物,通过水培实验,利用ATP酶抑制剂和离子通道抑制剂,探讨了香蒲根系吸收镉离子的途径,以及镉离子在香蒲根部的亚细胞分布特征和化学形态。研究结果显示:ATP酶抑制剂和非选择性阳离子通道抑制剂对香蒲吸收镉离子有显著的抑制作用(P<0.05);K+通道抑制剂对香蒲根吸收镉元素并未有显著影响;香蒲对镉离子的吸收存在主动吸收的可能性,香蒲可能通过阳离子通道吸收镉离子,但与K+通道无关;香蒲根部亚细胞中,镉的浓度大小顺序为:细胞壁>细胞器>液泡,香蒲根细胞中镉形态以氯化钠提取态为主,香蒲根细胞可将大部分镉隔离在细胞壁中,香蒲的细胞壁是其体内累积镉的一个重要位点,香蒲根部细胞壁的区隔化作用是其对镉富集解毒的重要生理机制之一。
Typhaangustifolia had been verified to be an enrichment plant of cadmium element,and a series of hydroponic culture experiments were carried out to study the relationship of Cd2 ＋uptake with different inhibitors of ion channels and energy consumption using ATP enzyme inhibitor and cationic ion channel blocker and potassium ion channel blocker. The combination forms of cadmium at cellular levels and chemical forms were also investigated. The results showed that ATP enzyme inhibitor and cationic ion channel blocker significantly limited the Cd uptake（ P 0. 05）. Conversely,K＋channel blocker did not inhibit Cd uptake,which showed that the Cd uptake in Typhaangustifolia was an active process and related to cationic ion channel,and the uptake of Cd in Typhaangustifolia was unrelated to K＋channel. The distributions of Cd concentration were different in Typhaangustifolia root cells： cellwall cellorgan vacuole. A high proportion of the sodium chloride extraction of cadmium were observed in the roots of Typhaangustifolia with cadmium treatment. Cellwall separation was one of the important physiological mechanism for the enrichment of cadmium detoxification of Typhaangustifolia.
出处
《环境工程》
CAS
CSCD
北大核心
2016年第8期58-61,共4页
Environmental Engineering
基金
广西科学研究与技术开发计划项目(桂科攻14124004-3-7)
广西高等学校高水平创新团队及卓越学者计划项目(002401013001)
关键词
香蒲
镉
吸收
化学形态
Typhaangustifolia
cadmium
uptake
chemical form
