摘要
目的 ：建立测定和胃化滞丸中黄芩苷含量的高效液相色谱法。方法：采用Thermo C18色谱柱（100 mm×4.6 mm,5μm）,流动相为甲醇-水-磷酸（47∶53∶0.2）,流速为1.0 m L/min,检测波长为280 nm,柱温为36℃。结果：黄芩苷对照品溶液在12-96μg/m L（r=0.999 4）浓度范围内与峰面积呈良好的线性关系,平均回收率为98.8%,RSD=0.59%（n=6）。结论：该法可用于和胃化滞丸中黄芩苷的含量测定。
Objective ：To develop a method for determination of baicalin conteint in hewei huazhi pills. Methods：A Ther mo Cl8column（100 mm × 4.6 mm,5 μm） was adopted, using methanol-water-phosphoric acid（47∶53∶0.2） as the mobile phase, at a flow rate of 1.0 m L/min, a detection wavelength of 280 nm and a temperature of 36 ℃. Results：Baicalin at a concentration of 12 -96 μg/m L showed good linear relationship to the peak area. The average recovery was 98.8 %,with a RSD of 0.59%（n = 6）. Conclusion：The method may be used for the determination of baicalin content in hewei huazhi pills.
出处
《中国执业药师》
CAS
2016年第8期32-34,共3页
China Licensed Pharmacist
