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ED1和CGRP在两种骨关节炎模型的表达及临床意义

Significance of the Clinic and Expression of ED1,CGRP between Operated and Running Exercise to Osteoarthritis Animal Model in Wistar Rats
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摘要 研究在不同骨关节炎中单克隆抗体(ED1)阳性细胞及降钙素基因相关肽(CGRP)阳性神经纤维在骨关节炎(OA)各个相同时期的表达变化及临床意义。将45只健康成年Wistar雄性大鼠随机分为正常组、手术组、跑步训练组,并进行OA造模。分别于1周、2周、3周、6周时处死大鼠,从各组对应时期取5只大鼠全膝关节组织置于4%PFA液固定、制片、免疫染色后,采用病理图像分析系统,通过观察及半定量ED1阳性细胞和CGRP阳性神经纤维的表达,评价两种骨关节炎中滑膜组织的早期病理变化。手术组及跑步训练组在1、2、3周较正常组均可见较多的ED1阳性细胞,手术组ED1阳性细胞升高明显,且手术组和跑步训练组在1、2、3周时ED1阳性细胞数呈极显著差异(P<0.01)。CGRP阳性神经纤维的表达显示:跑步训练组增多显著;2周时两组的CGRP阳性神经纤维数存在显著或极显著差异(P<0.05或0.01)。手术组的ED1阳性细胞的表达明显高于跑步训练组;而CGRP阳性神经纤维的表达显示跑步训练组高于手术组。 To investigate the expression of ED1 and calcitonin gene related peptide (CGRP) between the operation and running exercise for deferential osteoarthritis animal model, 45 male Wistar rats were randomly divided into 3 groups:control group (A), operation group (B) and running exercise group (C). The whole knee osteoarthritis of model rats were collected at 1, 2, 3 and 6 weeks and fixed in 4% PFA solution, section, immunohistochemical staining. The expression of ED1, CGRP and the pathological changes of the early osteoarthritis (OA) synovium were observed. Compared with group B, CGRP positive never fibers(7.753±0.736), (2.214±1.021) to group C was lighter (P〈0.05);ED1 positive cells expression(401.771±5.632), (353.440±4.321), (132.302±3.447)and(5.562±1.201), (18.665±1.533), (11.629±1.217)significantly higher (P〈0.01 or 0.05). ED1 positive cells expression in the group B is higher than that in group C;but Running exercise group.CGRP positive never fibers are higher than the operation group.
出处 《云南农业大学学报(自然科学版)》 CSCD 北大核心 2016年第4期637-643,共7页 Journal of Yunnan Agricultural University:Natural Science
基金 云南省应用基础研究计划项目(2013FZ182) 云南省应用基础研究(昆医联合专项)基金资助项目(2015FB095)
关键词 骨关节炎 滑膜组织 单克隆抗体 降钙素基因相关肽 osteoarthritis synovium ED1 calcitonin gene related peptide (CGRP)
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参考文献12

  • 1袁望舒,陈丽霞,刘淑芬.负重游泳运动对膝骨性关节炎大鼠关节软骨及血清超氧化物歧化酶和丙二醛的影响[J].中华物理医学与康复杂志,2014,36(2):81-85. 被引量:4
  • 2李宁华,张耀南,张毅,王坤正,李恩,朱汉民,金大地,陶天遵,杨静,薛庆云,黄公怡.国内六大行政区域六城市中老年人群膝关节骨性关节炎患病危险因素比较[J].中国组织工程研究与临床康复,2007,11(39):7758-7760. 被引量:131
  • 3梁永生,李方祥,解强,周敬滨,崔颖,孙贵先.备战第28届奥运会中国优秀运动员运动损伤调查分析[J].中国运动医学杂志,2007,26(6):744-745. 被引量:17
  • 4ROGART J N,BARRACH H J,CHICHESTER C O.Articular collagen degradation in the Hulth-telhag model of osteoarthritis[J].Osteoarthritis and Cartilage,1999,7(6):539.
  • 5SEKIYA I,TANG T,HAYASHI M,et al.Periodic knee injections of BMP-7 delay cartilage degeneration induced by excessive running in rats[J].Journal of Orthopaedic Research,2009,27(8):1088.
  • 6TANG T,MUNETA T,JU Y J,et al.Serum keratan sulfate transiently increases in the early stage of osteoarthritis during strenuous running of rats:protective effect of intraarticular hyaluronan injection[J].Arthritis Research&Therapy,2008,10(1):1.
  • 7TANG T,MUNETA T,SEKIYA I.Fibrous change of the infrapatellar fat pad due to strenuous running exercise and its treatment with intraarticular hyaluronan injection in a rat model[J].Journal of Medical and Dental Sciences,2008,55(1):163.
  • 8DIJKSTRA C D,D?PP E A,JOLING P,et al.The heterogeneity of mononuclear phagocytes in lymphoid organs:distinct macrophage subpopulations in the rat recognized by monoclonal antibodies ED1,ED2 and ED3[J].Immunology,1985,54(3):589.
  • 9MANKIN H J,DORFMAN H,LIPPIELLO L,et al.Biochemical and metabolic abnormalities in articular cartilage from osteo-arthritic human hips.Ⅱ.Correlation of morphology with biochemical and metabolic data[J].The Journal of Bone and Joint Surgery,1971,53(3):523.
  • 10孙先润,唐涛,李跃琼,许翔英,叶吉云,段艳萍,高国玺,赵晓芳,马骏,郭伟.SD大鼠膝前交叉韧带切断后骨关节的变化[J].中国矫形外科杂志,2013,21(22):2294-2298. 被引量:14

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